Valadbeigi Shirin, Saghiri Reza, Ebrahimi-Rad Mina, Khatami Shohreh, Akhbari Hadi
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Department of Rheumatology, Medical Sciences University of Birjand, Birjand, Iran.
Curr Rheumatol Rev. 2019;15(1):44-49. doi: 10.2174/1573397114666180406101239.
Rheumatoid Arthritis (RA) is a chronic multi systemic disorder with the unclarified ethiopathology. Although several markers have been presented for recognition of RA, but none of them has been specific. New markers such as HLA typing and activity of Adenosine Deaminase (ADA) isoenzymes could be useful and specific.
The aim of this study is to evaluate the pattern of ADA isoenzymes activity and HLA typing in both RA patients and healthy cases.
Blood samples were collected from 55 RA patients and 60 healthy subjects, over a period of 6 months. Levels of C-reactive Protein (CRP), Rheumatoid Factor (RF) and ADA (ADA1, ADA2, total ADA) were measured using AVITEX kit and HITACHI Auto Analyzer. In addition, HLA-DRB1*01,*04 and *10 was detected using PCR-SSP.
ADA activity, particularly ADA2 level, was significantly higher among RA group (Pv <0.05). The concentrations of tADA in patients with RF and CRP positive were significantly higher (Pv <0.05). The allele prevalence of DRB1*01 was significantly higher in RA patients (13.1%) compared with control group (5.5%, respectively) (P <0.05, Bonferroni adjustment P<0.003). Calculated sensitivity and specificity for diagnostic tests in this study are listed as: CRP (75%), RF (80%), ADA (84%) and RF (90%), ADA (83%), CRP (72%), respectively.
Increased tADA level and the frequency of DRB1*10 and *01 caused susceptibility to RA.
类风湿关节炎（RA）是一种慢性多系统疾病，其发病机制尚不明确。尽管已经提出了几种用于识别RA的标志物，但没有一种是特异性的。新的标志物如HLA分型和腺苷脱氨酶（ADA）同工酶活性可能是有用且特异的。
本研究旨在评估RA患者和健康对照者中ADA同工酶活性模式和HLA分型情况。
在6个月的时间里，收集了55例RA患者和60例健康受试者的血样。使用AVITEX试剂盒和日立自动分析仪测量C反应蛋白（CRP）、类风湿因子（RF）和ADA（ADA1、ADA2、总ADA）水平。此外，使用PCR-SSP检测HLA-DRB1*01、04和10。
RA组的ADA活性，尤其是ADA2水平显著更高（P<0.05）。RF和CRP阳性患者的总ADA浓度显著更高（P<0.05）。RA患者中DRB1*01的等位基因频率显著高于对照组（分别为13.1%和5.5%）（P<0.05，Bonferroni校正P<0.003）。本研究中诊断试验的计算敏感性和特异性分别列为：CRP（75%）、RF（80%）、ADA（84%）以及RF（90%）、ADA（83%）、CRP（72%）。
总ADA水平升高以及DRB110和01频率增加导致对RA易感。
