Tseng Te-Yu, Lai Pau-Jen, Sung Kung-Bin
Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan.
Opt Express. 2011 Jan 17;19(2):1291-300. doi: 10.1364/OE.19.001291.
To facilitate the application of plasmonic nanoparticles (PNPs) in high-throughput detection, we develop a hyperspectral imaging system (HSIS) combining dark-filed microscopy and imaging Fourier transform spectrometry to measure scattering spectra from immobilized PNPs. The current setup has acquisition time of 5 seconds and spectral resolution of 21.4 nm at 532.1 nm. We demonstrate the applicability of the HSIS in conjunction with spectral data analysis to quantify multiple types of PNPs and detect small changes in localized surface plasmon resonance wavelengths of PNPs due to changes in the environmental refractive index.
为促进等离子体纳米颗粒（PNPs）在高通量检测中的应用，我们开发了一种结合暗场显微镜和成像傅里叶变换光谱仪的高光谱成像系统（HSIS），以测量固定化PNPs的散射光谱。当前设置的采集时间为5秒，在532.1nm处的光谱分辨率为21.4nm。我们证明了HSIS结合光谱数据分析在量化多种类型PNPs以及检测由于环境折射率变化导致的PNPs局域表面等离子体共振波长的微小变化方面的适用性。
