Centre for NanoScale Science and Nanotechnology, School of Chemical and Physical Sciences, Flinders University, Adelaide SA 5001, Australia.
Biomicrofluidics. 2010 Dec 6;4(4):46504. doi: 10.1063/1.3523055.
Here, we present a simple chemical modification of poly(dimethylsiloxane) (PDMS) by curing a mixture of 2 wt% undecylenic acid (UDA) in PDMS prepolymer on a gold-coated glass slide. This gold slide had been previously pretreated with a self-assembled hydrophilic monolayer of 3-mercaptopropionic acid (MPA). During curing of the UDA∕PDMS prepolymer, the hydrophilic UDA carboxyl moieties diffuses toward the hydrophilic MPA carboxyl moieties on the gold surface. This diffusion of the UDA within the PDMS prepolymer to the surface is a direct result of surface energy minimization. Once completely cured, the PDMS is peeled off the gold substrate, thereby exposing the interfacial carboxyl groups. These groups are then available for subsequent attachment of 5(')-amino terminated DNA oligonucleotides via amide linkages. Our results show that the covalently tethered oligonucleotides can successfully capture fluorescein-labeled complementary oligonucleotides via hybridization, which are visualized using fluorescence microscopy.
在这里，我们通过在金涂覆玻璃载玻片上固化 PDMS 预聚物中 2wt%十一烯酸（UDA）的混合物，展示了一种对聚二甲基硅氧烷（PDMS）的简单化学修饰。该金载玻片之前已用 3-巯基丙酸（MPA）的自组装亲水单层预处理。在 UDA∕PDMS 预聚物的固化过程中，亲水的 UDA 羧基部分向金表面上的亲水 MPA 羧基部分扩散。UDA 在 PDMS 预聚物内扩散到表面是表面能最小化的直接结果。一旦完全固化，PDMS 就从金基底上剥离，从而暴露出界面羧基。这些基团随后可通过酰胺键与 5'（-）氨基末端 DNA 寡核苷酸进行后续连接。我们的结果表明，通过杂交可以成功地用共价键固定的寡核苷酸捕获荧光素标记的互补寡核苷酸，并用荧光显微镜观察到。
