Wetzstein M, Schumann W
Lehrstuhl für Genetik, Universität Bayreuth, F.R.G.
FEMS Microbiol Lett. 1990 Oct;60(1-2):55-8. doi: 10.1016/0378-1097(90)90344-p.
To find out whether Escherichia coli heat shock promoters are recognized in Bacillus subtilis, the regulatory regions including the heat shock promoters of the main heat shock genes dnaKJ, lon, groES, and htpG were fused to the indicator genes lacZ and cat. Whereas all transcriptional fusions were expressed in E. coli at low temperature and transient increases of beta-galactosidase activity could be measured at the inducing temperature, no enzymatic activity was found in B. subtilis. This indicates that E. coli heat shock promoters are nonfunctional in B. subtilis.
为了确定枯草芽孢杆菌是否能识别大肠杆菌的热休克启动子，将包括主要热休克基因dnaKJ、lon、groES和htpG的热休克启动子在内的调控区域与报告基因lacZ和cat融合。所有转录融合体在大肠杆菌中于低温下表达，并且在诱导温度下可检测到β-半乳糖苷酶活性的短暂增加，而在枯草芽孢杆菌中未发现酶活性。这表明大肠杆菌的热休克启动子在枯草芽孢杆菌中无功能。
