Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
J Microencapsul. 2011;28(2):99-107. doi: 10.3109/02652048.2010.534824.
The treatment of osteomyelitis remains a challenge for orthopaedic surgeons. Controlled release of vancomycin from biodegradable microspheres is a promising method for eliminating infection. However, the large initial burst release may make it difficult to maintain the local vancomycin concentration superior to minimum inhibitory concentration for several weeks. The aims of this study were to explore applications of the silk fibroin (SF) as an aqueous coating material for vancomycin-loaded poly(ε-caprolactone) (PCL) microspheres, and investigate the effects of silk coating on in vitro drug release. Examinations of particle size analyses, vancomycin content, Fourier transform infrared spectroscopy, differential scanning calorimetry, scanning electron microscopy and in vitro drug release were performed. The results showed that silk coating could reduce the large initial burst release and retard the vancomycin release. Therefore, we suggest that the SF could be used as an aqueous coating material for vancomycin-loaded PCL microspheres and prolonged the drug release. SF coating on vancomycin-loaded PCL microspheres may be considered as an effective approach to prolong the drug release and improve the anti-infection effects.
骨髓炎的治疗仍然是骨科医生面临的挑战。从生物可降解微球中释放万古霉素是消除感染的一种很有前途的方法。然而，大的初始突释可能使得难以维持局部万古霉素浓度超过最低抑菌浓度数周。本研究的目的是探索丝素（SF）作为万古霉素载聚己内酯（PCL）微球的水包衣材料的应用，并研究丝素包衣对体外药物释放的影响。进行了粒径分析、万古霉素含量、傅里叶变换红外光谱、差示扫描量热法、扫描电子显微镜和体外药物释放的检测。结果表明，丝素包衣可以减少大的初始突释并延缓万古霉素的释放。因此，我们建议 SF 可以用作万古霉素载 PCL 微球的水包衣材料，并延长药物释放。SF 包被的万古霉素载 PCL 微球可能被认为是一种延长药物释放和提高抗感染效果的有效方法。
