Department of Biology (Area 5), University of York, York YO10 5DD, UK.
Biochem Soc Trans. 2011 Jan;39(1):111-5. doi: 10.1042/BST0390111.
Methanococcus maripaludis S2 is a methanogenic archaeon with a well-developed genetic system. Its mesophilic nature offers a simple system in which to perform complementation using bacterial and eukaryotic genes. Although information-processing systems in archaea are generally more similar to those in eukaryotes than those in bacteria, the order Methanococcales has a unique complement of DNA replication proteins, with multiple MCM (minichromosome maintenance) proteins and no obvious originbinding protein. A search for homologues of recombination and repair proteins in M. maripaludis has revealed a mixture of bacterial, eukaryotic and some archaeal-specific homologues. Some repair pathways appear to be completely absent, but it is possible that archaeal-specific proteins could carry out these functions. The replication, recombination and repair systems in M. maripaludis are an interesting mixture of eukaryotic and bacterial homologues and could provide a system for uncovering novel interactions between proteins from different domains of life.
产甲烷八叠球菌 S2 是一种产甲烷古菌，具有发达的遗传系统。其嗜中性使其成为一个简单的系统，可以使用细菌和真核基因进行互补。尽管古菌中的信息处理系统通常与真核生物的系统更相似，而不是与细菌的系统相似，但 Methanococcales 目具有独特的 DNA 复制蛋白组成，具有多个 MCM（微染色体维持）蛋白，而没有明显的起始结合蛋白。在产甲烷八叠球菌中搜索重组和修复蛋白的同源物，揭示了细菌、真核生物和一些古菌特异性同源物的混合物。一些修复途径似乎完全缺失，但有可能是古菌特异性蛋白可以执行这些功能。产甲烷八叠球菌的复制、重组和修复系统是真核生物和细菌同源物的有趣组合，可能为揭示来自不同生命领域的蛋白质之间的新相互作用提供系统。
