Kehrer G, Blech M, Kallerhoff M, Kleinert H, Langheinrich M, Bretschneider H J
Department of Physiology and Pathophysiology, University of Göttingen, FRG.
J Invest Surg. 1990;3(2):147-68. doi: 10.3109/08941939009140345.
In ischemic canine kidneys protected by Bretschneider's HTK solution the glycolytic lactate production is limited by a low renal substrate content. However, for anaerobic energy supply ischemic organs depend on glycolysis. To evaluate the role of glycolysis in renal protection, the relationship between lactate production and anaerobic energy supply was examined in protected kidneys of dogs, sheep, and swine. Additionally, in canine kidneys an attempt was made to improve anaerobic energy provision by adding glucose to the protective solution. The results were as follows: (1) According to increasing lactate production from swine to dog to sheep, intraischemic ATP decay was delayed least in swine and most in sheep. (2) Glucose addition (10 mM) to the HTK solution roughly doubled the time for ATP to fall to 1 mumol/g dry wt (tATP) in dogs. (3) The greater the lactate production in all three species, the lower the decrease in SAN (ATP + ADP + AMP) from 5 to 120 min of ischemia. (4) A glucose additive in the protective solution led to a significant (p less than .005) increase of SAN in dogs at 120 min of ischemia. A sufficient substrate supply seems to be an essential component of a reliable renal protection.
在由布雷施奈德氏HTK溶液保护的缺血犬肾中，糖酵解乳酸生成受到低肾底物含量的限制。然而，对于无氧能量供应，缺血器官依赖糖酵解。为了评估糖酵解在肾脏保护中的作用，研究了犬、羊和猪的受保护肾脏中乳酸生成与无氧能量供应之间的关系。此外，在犬肾中尝试通过向保护溶液中添加葡萄糖来改善无氧能量供应。结果如下：(1) 随着从猪到犬再到羊乳酸生成的增加，缺血期间ATP衰减延迟程度在猪中最小，在羊中最大。(2) 向HTK溶液中添加葡萄糖（10 mM）使犬肾中ATP降至1 μmol/g干重（tATP）的时间大致增加了一倍。(3) 在所有三个物种中，乳酸生成越多，缺血5至120分钟期间SAN（ATP + ADP + AMP）的下降幅度越小。(4) 在缺血120分钟时，保护溶液中的葡萄糖添加剂导致犬肾中SAN显著增加（p <.005）。充足的底物供应似乎是可靠肾脏保护的一个重要组成部分。
