Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida 32080-8610, USA.
J Cell Biochem. 2011 Feb;112(2):541-7. doi: 10.1002/jcb.22944.
The mouse COL3A1 first intron is 9684 bp. RNA's of approximately 1.6 and 3.0 kb were detected by Northern hybridization analysis of poly-A RNA from fetal mice and total RNA from suckling and adult mouse intestine using (32)P-labeled, anti-sense RNA synthesized from a mouse COL3A1 first intron, 5 prime region, 5.4 kb Xba I fragment (1655-7030 bp), recombinant plasmid (pPI5.4x). Expression of the 1.6 and 3.0 kb RNA's was significantly reduced in adult mouse intestine, indicating that these RNAs are developmentally regulated. "BLAST" analysis indicated that the mouse first intron 5 prime sequence has 94-100% identity to 13 mouse ESTs. These mouse first intron EST's lie within the 5.4 Xba I fragment of the mouse COL3A1 first intron. Two of the mouse first intron EST's have significant identity to known miRNA, mature sequences, mmu-miR-466f-3P, mmu-miR-1187, and mmu-miR-574-5P as well as others. Predicted targets for mmu-miR-466f-3P include COL1A1, COL19A1, COL11A2, COL4A1, and COL4A5 indicating that COL3A1 intronic miRNAs may regulate the expression of other collagen genes in development.
该鼠标 COL3A1 第一内含子是 9684bp。 RNA 的大约 1.6 和 3.0kb 检测到通过 Northern 杂交分析多聚 - A RNA 从胎儿小鼠和总 RNA 从哺乳期和成年小鼠肠使用（32）P 标记，反义 RNA 合成从鼠标 COL3A1 第一内含子，5 端，5.4kb Xba I 片段（1655-7030bp），重组质粒（pPI5.4x）。表达 1.6 和 3.0kb RNA 的是明显减少成年小鼠肠道，表明这些 RNA 是发育调控。 “BLAST”分析表明，鼠标第一内含子 5 端序列有 94-100%的同一性 13 鼠标 ESTs。这些鼠标第一内含子 EST 的谎言内 5.4 Xba I 片段的鼠标 COL3A1 第一内含子。两个鼠标第一内含子 EST 的有显著的同一性已知 miRNA，成熟序列，mmu-miR-466f-3P，mmu-miR-1187，和 mmu-miR-574-5P 以及其他。预测的目标 mmu-miR-466f-3P 包括 COL1A1、COL19A1、COL11A2、COL4A1 和 COL4A5 表明 COL3A1 内含子 miRNA 可能调节表达的其他胶原蛋白基因的发育。
