Zhang Q J, Liu Z Y
Institute of Biophysics, Chinese Academy of Sciences, Beijing.
Prep Biochem. 1990;20(3-4):257-62. doi: 10.1080/00327489008050200.
A novel method for isolation and concentration of RNase T1 from Taka-Diastase is developed. It is a combination method of bentonite adsorption with dialysis desorption. In the present method, RNase T1 can be concentrated about ten-fold, the recovery of total activity was greater than 95%, and specific activity was raised 8-10 folds. Further purification with ammonium sulfate precipitation and chromatography on DEAE-cellulose and DEAE-Sephadex yields a RNase T1 which contains no pMase. pDase nor RNase T2 activities and a 750 fold increase in specific activity. Our method is more simple, rapid, and efficient than previous methods.
开发了一种从高峰淀粉酶中分离和浓缩核糖核酸酶T1的新方法。它是膨润土吸附与透析解吸的组合方法。在本方法中，核糖核酸酶T1可浓缩约10倍，总活性回收率大于95%，比活性提高8 - 10倍。通过硫酸铵沉淀以及在DEAE - 纤维素和DEAE - 葡聚糖凝胶上进行色谱进一步纯化，得到的核糖核酸酶T1不含有磷酸单酯酶、磷酸二酯酶和核糖核酸酶T2活性，比活性提高了750倍。我们的方法比以前的方法更简单、快速和高效。
