[人源微小RNA-126和人源微小RNA-518b在食管鳞状细胞癌中的差异表达]
[Differential expression of hsa-miR-126 and hsa-miR-518b in esophageal squamous carcinoma].
作者信息
Cai Hui, Wang Jian-sheng, Zhang Ming-xin, Duan Xiao-yi, Ma Ren-qiang
机构信息
Department of Oncology Surgery, Xi an Jiaotong University College of Medicine, Xi an 710061, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jan;31(1):23-7.
OBJECTIVE
To identify the differentially expressed microRNAs (miRNAs) between esophageal squamous carcinoma (ESC) and adjacent non-tumorous tissue (NT).
METHODS
The expression levels of the miRNAs were detected in 3 fresh ESC and NT samples by hybridization with miRNAs microarray chip. Real-time quantitative RT-PCR was performed to confirm the results of the microarray analysis. The expressions of hsa-miR-126 and hsa-miR-518b in ESC were validated by real-time quantitative RT-PCR in another independent 15 matched samples.
RESULTS
A total of 11 miRNAs exhibited differential expressions in ESC samples as compared to their expressions in the NT samples, including a 1 up-regulated miRNA and 10 down-regulated miRNAs. Compared with normal esophageal samples, the ESC tissues showed up-regulated hsa-miR-126 and down-regulated hsa-miR-518b expression.
CONCLUSION
hsa-miR-126 and hsa-miR-518b are differentially expressed in ESC, and they might play important roles in the carcinogenesis and progression of ESC.
目的
鉴定食管鳞状细胞癌（ESC）与癌旁非肿瘤组织（NT）之间差异表达的微小RNA（miRNA）。
方法
通过与miRNA微阵列芯片杂交，检测3份新鲜的ESC和NT样本中miRNA的表达水平。进行实时定量逆转录-聚合酶链反应（RT-PCR）以确认微阵列分析的结果。在另外15对匹配样本中，通过实时定量RT-PCR验证ESC中hsa-miR-126和hsa-miR-518b的表达。
结果
与NT样本中的表达相比，共有11种miRNA在ESC样本中表现出差异表达，包括1种上调的miRNA和10种下调的miRNA。与正常食管样本相比，ESC组织中hsa-miR-126表达上调，hsa-miR-518b表达下调。
结论
hsa-miR-126和hsa-miR-518b在ESC中差异表达，它们可能在ESC的发生和发展中起重要作用。
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