Department of Cardiothoracic Surgery, the First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, PR China.
J Surg Oncol. 2012 Feb;105(2):175-82. doi: 10.1002/jso.22066. Epub 2011 Aug 22.
microRNAs (miRNAs), small non-coding RNAs, are always aberrantly expressed in many diseases including human cancers. The aim of this study was to examine and determine the clinical significance of hsa-miR-31, hsa-miR-142-3p, hsa-miR-338-3p, and hsa-miR-1261 expression in esophageal squamous cell carcinoma (ESCC).
Expression levels of four selected miRNAs, initially evaluated by microarray, were validated by qRT-PCR. Various statistical methods were used to analyze the relationship between miRNA expression and clinicopathologic features and prognosis in 91 patients with ESCC.
MiR-31 and miR-142-3p expression were correlated to histological differentiation in ESCC (P < 0.05, Student's t-test); high miR-142-3p expression was associated with a poor prognosis in all 91 ESCC patients (P = 0.014, log-rank) and identified as an independent prognostic factor in ESCC (P = 0.017, univariate Cox; P = 0.022, multivariate Cox). More importantly, stratified analysis indicated that high miR-142-3p expression was correlated to a poor prognosis within good-prognosis groups comprised of ESCC patients with small tumor size, negative lymph node metastasis, or early stage (all P < 0.05).
The main findings suggest that miR-142-3p is involved in the progression of ESCC and is a potential prognostic biomarker for ESCC.
微小 RNA（miRNA）是一种小的非编码 RNA，在许多疾病中（包括人类癌症）经常出现异常表达。本研究旨在检测并确定 hsa-miR-31、hsa-miR-142-3p、hsa-miR-338-3p 和 hsa-miR-1261 在食管鳞状细胞癌（ESCC）中的表达及其临床意义。
通过微阵列初步评估了四个选定 miRNA 的表达水平，并用 qRT-PCR 进行验证。采用多种统计方法分析了 91 例 ESCC 患者 miRNA 表达与临床病理特征及预后的关系。
miR-31 和 miR-142-3p 的表达与 ESCC 的组织学分化有关（P<0.05，Student's t 检验）；miR-142-3p 高表达与所有 91 例 ESCC 患者的预后不良相关（P=0.014，log-rank 检验），并被确定为 ESCC 的独立预后因素（P=0.017，单因素 Cox；P=0.022，多因素 Cox）。更重要的是，分层分析表明，在肿瘤体积小、淋巴结转移阴性或早期的 ESCC 患者预后较好的亚组中，高 miR-142-3p 表达与预后不良相关（均 P<0.05）。
研究结果提示 miR-142-3p 参与 ESCC 的进展，是 ESCC 的潜在预后生物标志物。
