Xiao Hua-ping, Xiao Jin-fang, Gu Miao-ning
Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jan;31(1):171-4.
To investigate the effect of propofol on the proliferation and differentiation of rat embryonic neural stem cells in vitro.
Embryonic neural stem cells of fetal Wistar rats (gestational age of 14-16 days) in primary culture, after identification for nestin expression, were divided into control group, introlipid group, and propofol groups (treated with propofol at the doses of 5, 25, 50, and 100 µmol/L). The changes in the proliferation of the embryonic neural stem cells after the treatments were observed using Brdu incorporation assay. In the course of induced differentiation of the embryonic neural stem cells, 50 µmol/L propofol was added in the cells to assess its impact on the differentiation of the cells by immunohistochemical detection of NeuN and GFAP expressions.
More than 95% of the embryonic neural stem cells in primary culture were Nestin-positive. The percentages of Brdu-positive cells showed no significant changes after treatment with different concentrations of propofol, whereas the addition of 50 µmol/L propofol resulted in a significant increase of NeuN-positive cell percentage to (23.1∓0.9)% as compared with that of (13.4∓0.8)% in the control group (P<0.05) without affecting the GFAP-positive cells.
Clinically relevant doses of propofol have no obvious effect on the proliferation of rat neural stem cells cultured in vitro, but can induce their differentiation into neuron-like cells.
探讨丙泊酚对体外培养的大鼠胚胎神经干细胞增殖和分化的影响。
原代培养孕14 - 16天的Wistar大鼠胚胎神经干细胞，经巢蛋白表达鉴定后，分为对照组、脂肪乳剂组和丙泊酚组（分别用5、25、50和100 μmol/L丙泊酚处理）。采用溴脱氧尿苷掺入法观察处理后胚胎神经干细胞增殖的变化。在胚胎神经干细胞诱导分化过程中，向细胞中加入50 μmol/L丙泊酚，通过免疫组织化学检测NeuN和GFAP表达来评估其对细胞分化的影响。
原代培养的胚胎神经干细胞中95%以上为巢蛋白阳性。不同浓度丙泊酚处理后，溴脱氧尿苷阳性细胞百分比无显著变化，而加入50 μmol/L丙泊酚后，NeuN阳性细胞百分比显著增加至(23.1±0.9)%，对照组为(13.4±0.8)%（P<0.05），且不影响GFAP阳性细胞。
临床相关剂量的丙泊酚对体外培养的大鼠神经干细胞增殖无明显影响，但可诱导其分化为神经元样细胞。
