Mazurenko N N, Kiseleva N P, Kudriavets Iu I, Mikhaleva I I, Antonenko V V, Kiselev F L
Mol Biol (Mosk). 1990 Sep-Oct;24(5):1351-62.
Antibodies to c-fos oncoprotein were produced in rabbits by immunization with synthetic peptides, corresponding to the sequences 6-15 of N-end and 371-380 of C-end of c-fos oncoprotein. C-fos expression was tested with immunoprecipitation and immunoblotting in various transformed cell lines with antibodies to N- and C-decapeptides. It was shown that antibodies to C-terminal decapeptide revealed a c-fos gene product and also some fos-related antigens FRAs 36 kD, 46 kD, 75 kD and 90 kD in rat pheochromocytoma PC-12 cells and mouse carcinoma cell lines MAC-3 and LL. In some cell lines 46 kD FRA was expressed in the absence of p62 c-fos. Besides, different clones of the same cell line cultivated in identical conditions revealed differences in the 46 kD FRA expression. Antibodies to sequence 6-15 of N-end revealed only c-fos products and no FRAs were detected. Therefore FRAs have homology with the c-fos product in the C-terminal region and differ from it in the N-terminal region.
通过用与c-fos癌蛋白N端6 - 15序列和C端371 - 380序列相对应的合成肽对兔子进行免疫，制备出了抗c-fos癌蛋白的抗体。使用针对N端和C端十肽的抗体，通过免疫沉淀和免疫印迹法在各种转化细胞系中检测c-fos的表达。结果表明，针对C端十肽的抗体在大鼠嗜铬细胞瘤PC - 12细胞以及小鼠癌细胞系MAC - 3和LL中揭示了一种c-fos基因产物以及一些36 kD、46 kD、75 kD和90 kD的fos相关抗原（FRA）。在一些细胞系中，46 kD FRA在没有p62 c-fos的情况下表达。此外，在相同条件下培养的同一细胞系的不同克隆在46 kD FRA表达上存在差异。针对N端6 - 15序列的抗体仅检测到c-fos产物，未检测到FRA。因此，FRA在C端区域与c-fos产物具有同源性，而在N端区域与之不同。
