Feng Shi-Ting, Li Hao, Sun Can-Hui, Qiu Peng-Xin, Zhang Zhong-Wei, Shuai Xin-Tao, Li Zi-Ping, Meng Quan-Fei
Department of Radiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2011 Jan;14(1):27-30.
To study the feasibility of MRI of human colon adenocarcinoma cell line (Lovo) labeled with superparamagnetic iron oxide(SPIO) nanoparticles in vitro.
Lovo cells (5 × 10(5) and 1 × 10(6)) were cultured in medium containing different SPIO nanoparticles (50 microl and 500 microl). Transmission electron microscopy was used to observe cellular ultrastructure and to determine the uptake and distribution of particles in Lovo cells at 1-, 3-, 6-hours. MRI of Lovo cells was performed with T1WI, T2WI sequences. Unlabeled cells were used as controls.
Uptake of SPIO nanoparticles occurred within 6 hours. On T1 weighted imaging, there was no significant difference in signal intensity between the experimental groups and the control group. On T2 weighted imaging, there was no significant difference in signal intensity between the experimental groups and the control group after culture of 1 h. Signal intensity began to decrease in 1 × 10(6) Lovo cells labeled with 500 microl SPIO nanoparticle after 3 hours culture. Signal intensity decreased in all the experimental groups after 6 hours culture.
Human colon adenocarcinoma cell line (Lovo) can be labeled with SPIO nanoparticles, and the labeled cells can be imaged with MRI equipment.
研究超顺磁性氧化铁（SPIO）纳米颗粒标记人结肠腺癌细胞系（Lovo）用于体外磁共振成像（MRI）的可行性。
将Lovo细胞（5×10⁵和1×10⁶）培养于含不同SPIO纳米颗粒（50微升和500微升）的培养基中。采用透射电子显微镜观察细胞超微结构，并于1、3、6小时测定Lovo细胞中颗粒的摄取及分布情况。用T1WI、T2WI序列对Lovo细胞进行MRI检查。未标记细胞作为对照。
SPIO纳米颗粒在6小时内被摄取。在T1加权成像上，实验组与对照组信号强度无显著差异。在T2加权成像上，培养1小时后实验组与对照组信号强度无显著差异。培养3小时后，用500微升SPIO纳米颗粒标记的1×10⁶ Lovo细胞信号强度开始降低。培养6小时后所有实验组信号强度均降低。
人结肠腺癌细胞系（Lovo）可用SPIO纳米颗粒标记，且标记后的细胞可用MRI设备成像。
