Quality of Plant Products, Department of Crop Sciences, Faculty of Agricultural Sciences, Georg-August University of Göttingen, Göttingen, Germany.
Proteomics. 2011 Mar;11(5):972-85. doi: 10.1002/pmic.201000322. Epub 2011 Jan 27.
The effect of artificial Fusarium graminearum and F. culmorum infection at the level of the proteome on grains of naked barley (Hordeum vulgare subsp. nudum) was investigated in comparison to naturally infected samples. Fusarium infection in barley led to numerous host-specific biochemical responses. NEPHGE 2-D PAGE and MS were used to identify proteins that were differentially expressed in response to fungal infection and growing location of the plants. Moreover, the mycotoxin concentration of the grains was evaluated to characterize the infection degree. Inoculation of naked barley with Fusarium led to grain deoxynivalenol concentrations of up to 1.2 mg/kg. The carbon and nitrogen grain concentrations were not significantly changed after fungal infection, but differed between growing locations. Eleven proteins related to fungal infection were detected as were three proteins with effects based on growing location. These proteins belong to different protein groups involved in various cell functions: transcription regulation, defence response, nutrient reservoirs and starch biosynthesis. The results gave indications on plant defence strategies and changes as response to Fusarium infection in mature grains after a long infection period as well as being influenced by the growing location.
本研究调查了人工感染禾谷镰刀菌（ Fusarium graminearum ）和尖孢镰刀菌（ Fusarium culmorum ）对青稞（ Hordeum vulgare subsp. nudum ）籽粒蛋白质组水平的影响，并与自然感染的样品进行了比较。大麦感染镰刀菌会引发许多宿主特异性的生化反应。我们采用 NEPHGE 2-D PAGE 和 MS 来鉴定对真菌感染和植株生长位置有差异表达的蛋白质。此外，还评估了谷物中霉菌毒素的浓度，以表征感染程度。用镰刀菌接种青稞，导致谷物脱氧雪腐镰刀菌烯醇浓度高达 1.2mg/kg。真菌感染后，碳氮谷物浓度没有明显变化，但在不同的生长地点有所不同。检测到与真菌感染相关的 11 种蛋白质和 3 种基于生长位置的蛋白质。这些蛋白质属于不同的蛋白质组，参与各种细胞功能：转录调控、防御反应、营养库和淀粉生物合成。这些结果表明，在感染后期，成熟谷物中存在长期的真菌感染，并受到生长位置的影响，植物具有防御策略和变化。
