Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Biochem Biophys Res Commun. 2011 Feb 25;405(4):610-4. doi: 10.1016/j.bbrc.2011.01.077. Epub 2011 Jan 25.
Lymphatic endothelial cells in tumors (T-LECs) are considered to have different characteristics from LECs in non-tumor tissues (N-LECs). However, differences between the two types have not been well analyzed at molecular level. In this report, we performed differential proteome analysis of T-LEC and N-LEC models prepared by cultivation of LECs in tumor conditioned medium. By expression profiling of identified proteins using tissue microarrays, reticulocalbin-1 was found to be expressed in clinical specimen-derived T-LECs and lung cancer cells but not N-LECs. It is suggested that reticulocalbin-1 may be an important molecule in understanding T-LEC function and control of lymphatic metastasis.
肿瘤中的淋巴管内皮细胞（T-LEC）被认为具有与非肿瘤组织中的淋巴管内皮细胞（N-LEC）不同的特征。然而，这两种类型在分子水平上的差异尚未得到很好的分析。在本报告中，我们通过在肿瘤条件培养基中培养 LEC 来制备 T-LEC 和 N-LEC 模型，并对其进行了差异蛋白质组分析。通过使用组织微阵列对鉴定出的蛋白质进行表达谱分析，发现网钙蛋白-1在源自临床标本的 T-LEC 和肺癌细胞中表达，但在 N-LEC 中不表达。提示网钙蛋白-1可能是理解 T-LEC 功能和控制淋巴转移的重要分子。
