插入肽的支撑混合双层膜的中子反射测量法
Neutron reflectometry of supported hybrid bilayers with inserted peptide.
作者信息
Smith Matthew B, McGillivray Duncan J, Genzer Jan, Lösche Mathias, Kilpatrick Peter K
机构信息
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA.
出版信息
Soft Matter. 2010;6(5):862-865. doi: 10.1039/b915800f.
Abstract
The insertion of a synthetic amphiphilic, α-helical peptide into a supported hybrid bilayer membrane (HBM) was studied by neutron reflectometry to elucidate the resulting nanostructure. The HBM consisted of a self-assembled monolayer of perdeuterated octadecanethiol on gold and an overlying leaflet of acyl-deuterated phosphatidylcholine (d-DMPC). Using contrast variation, several reflectivity spectra were recorded for each step of film fabrication, and simultaneously modeled. This analysis indicated that peptide insertion into the DMPC lipid leaflet is the likeliest mode of incorporation.
摘要
通过中子反射测量法研究了一种合成两亲性α-螺旋肽插入支撑混合双层膜（HBM）的过程，以阐明由此产生的纳米结构。HBM由金上的全氘代十八烷硫醇自组装单层和酰基氘代磷脂酰胆碱（d-DMPC）的上层小叶组成。利用对比度变化，在膜制备的每个步骤记录了几个反射光谱，并同时进行建模。该分析表明，肽插入DMPC脂质小叶是最有可能的掺入模式。
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2
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3
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