Department of Biotechnology, University of Turku, Turku, Finland.
Anal Chem. 2011 Feb 15;83(4):1456-61. doi: 10.1021/ac103155f. Epub 2011 Jan 24.
We have developed a robust array-in-well test platform based on an oligonucleotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.
我们开发了一种基于寡核苷酸微阵列的强大微孔板内检测平台，结合了仪器简单和新型上转换荧光报告物技术的优势。上转换无机镧系荧光粉在近红外激发下具有独特的可见波长光致发光发射特性。样品或支持材料不会产生自发荧光，从而实现了高灵敏度的检测。在这项研究中，检测是在标准的 96 孔微量滴定板中进行的，使得该技术很容易适用于高通量分析。寡核苷酸微孔板内检测用于检测引起人类感染的十种常见腺病毒基因型。该研究展示了基于上转换荧光报告物技术在多分析物检测和反斯托克斯光致发光检测中的优势和潜力，使用了反斯托克斯光致发光成像设备。
