School of Life Science, Beijing Institute of Technology, Beijing, China.
FEMS Microbiol Lett. 2011 Apr;317(2):203-10. doi: 10.1111/j.1574-6968.2011.02230.x. Epub 2011 Feb 22.
The Cry8Ea1 protoxin is a DNA-protein complex. Both forms of the Cry8Ea1 toxin (with or without DNA binding) were obtained separately, and their stability and ability to insert into a phospholipid monolayer in vitro were compared. The presence of DNA can prevent the toxin from aggregation. Data regarding the penetration of the Cry8Ea1 toxin and Cry8Ea1 toxin-DNA complex into the air/water interface without a phospholipid monolayer show that the Cry8Ea1 toxin-DNA complex is more likely to move towards the air/water interface and is more hydrophobic. Experiments examining the protein interaction with the phospholipid monolayer show that the ability of the Cry8Ea1 toxin-DNA complex to insert into the lipid bilayer is much greater than that of the Cry8Ea1 toxin without DNA.
Cry8Ea1 原毒素是一种 DNA-蛋白复合物。分别获得了具有或不具有 DNA 结合的两种形式的 Cry8Ea1 毒素，并比较了它们在体外的稳定性和插入磷脂单层膜的能力。DNA 的存在可以防止毒素聚集。关于 Cry8Ea1 毒素和 Cry8Ea1 毒素-DNA 复合物在没有磷脂单层膜的情况下穿透空气/水界面的数据表明，Cry8Ea1 毒素-DNA 复合物更有可能向空气/水界面移动，并且更具疏水性。研究蛋白质与磷脂单层膜相互作用的实验表明，Cry8Ea1 毒素-DNA 复合物插入脂质双层的能力远大于没有 DNA 的 Cry8Ea1 毒素。
