Bridelli M G, Crippa P R, Ugozzoli F
Department of Physics, University of Parma, Italy.
Pigment Cell Res. 1990 Oct;3(4):187-91. doi: 10.1111/j.1600-0749.1990.tb00288.x.
A series of experiments was performed on lyophylized melanosomes in order to analyze the melanin in the natural state as polymerized into these organelles and to verify in such biological amorphous material the possibility of obtaining intensity scattering curves from which Bragg distances can be calculated. The results confirm the feasibility of the method and show that melanins in melanosomes maintain many structural features of the purified form and that the biochemical composition of the organelles can be responsible for the observed differences in the diffractograms. The presence in melanosomes of supramolecular paracrystalline aggregates was also clearly demonstrated.
为了分析聚合到这些细胞器中的天然状态的黑色素，并在这种生物无定形材料中验证获得强度散射曲线的可能性，以便从中计算布拉格距离，对冻干的黑素小体进行了一系列实验。结果证实了该方法的可行性，并表明黑素小体中的黑色素保持了纯化形式的许多结构特征，并且细胞器的生化组成可能是衍射图中观察到的差异的原因。还清楚地证明了黑素小体中存在超分子准晶聚集体。
