Department of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR China.
FEBS Lett. 2011 Feb 18;585(4):657-63. doi: 10.1016/j.febslet.2011.01.027. Epub 2011 Jan 26.
There is increasing evidence that microRNAs (miRNAs) play important roles in cell proliferation, apoptosis and differentiation that accompany inflammatory responses. However, whether microRNAs are associated with DC immuno-inflammatory responses with oxidized low density lipoprotein (oxLDL) stimulation is not yet known. Our study aims to explore the link of miRNAs with lipid-overload and immuno-inflammatory mechanism for atherosclerosis. In DCs transfected with microRNA-29a mimics or inhibitors, we showed that microRNA-29a plays an important role in proinflammatory cytokine secretion and scavenger receptor expression upon oxLDL-treatment. Furthermore, we suggest an additional explanation for the mechanism of microRNA-29a regulation of its functional target, lipoprotein lipase. We conclude that microRNA-29a could regulate pro-inflammatory cytokine secretion and scavenger receptor expression by targeting lipoprotein lipase in oxLDL-stimulated dendritic cells.
越来越多的证据表明，微小 RNA（miRNA）在细胞增殖、凋亡和分化中发挥重要作用，这些过程伴随着炎症反应。然而，miRNA 是否与 DC 的免疫炎症反应有关，尤其是与氧化型低密度脂蛋白（oxLDL）刺激有关，目前尚不清楚。本研究旨在探讨 miRNA 与脂质过载和动脉粥样硬化免疫炎症机制的联系。在经 microRNA-29a 模拟物或抑制剂转染的 DC 中，我们表明 microRNA-29a 在 oxLDL 处理时对促炎细胞因子分泌和清道夫受体表达起着重要作用。此外，我们为 microRNA-29a 调节其功能靶标脂蛋白脂酶的机制提供了一个额外的解释。我们的结论是，microRNA-29a 可以通过靶向 oxLDL 刺激树突状细胞中的脂蛋白脂酶来调节促炎细胞因子分泌和清道夫受体表达。
