SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
FEBS Lett. 2011 Feb 18;585(4):683-6. doi: 10.1016/j.febslet.2011.01.032. Epub 2011 Jan 26.
APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The removal of the 3'-phosphate is performed by polynucleotide kinase phosphatase (PNKP). Tdp1 is known to interact stably with BER proteins: DNA polymerase beta (Pol β), XRCC1, PARP1 and DNA ligase III. The data suggest a role of Tdp1 in the new APE-independent BER pathway in mammals.
APE 非依赖型碱基切除修复（BER）途径在调控 DNA 修复机制方面发挥着重要作用。在本研究中发现，新近发现的酪氨酰-DNA 磷酸二酯酶 1（Tdp1）可催化 AP 位点的切割反应，生成具有 3'-和 5'-磷酸末端的断裂。3'-磷酸的去除由多核苷酸激酶磷酸酶（PNKP）完成。已知 Tdp1 可与 BER 蛋白（DNA 聚合酶β（Polβ）、XRCC1、PARP1 和 DNA 连接酶 III）稳定相互作用。这些数据表明 Tdp1 在哺乳动物中新的 APE 非依赖型 BER 途径中发挥作用。
