弗兰克氏菌 R43 氢化酶在不同丙酸碳源水平下的产氢量。

Hydrogen yield from a hydrogenase in Frankia R43 at different levels of the carbon source propionate.

机构信息

Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-901 87 Umeå, Sweden.

出版信息

J Environ Manage. 2012 Mar;95 Suppl:S365-8. doi: 10.1016/j.jenvman.2011.01.005. Epub 2011 Jan 26.

DOI:10.1016/j.jenvman.2011.01.005

Abstract

Fermentative hydrogen yield was investigated in the Frankia strain R43, which was grown in different amounts of the carbon source propionate. In relation to hydrogen yield, the hydrogenase enzyme was characterized by use of Western blot. A bioreactor study revealed a 10-fold increase in growth within 50 h. The study showed that there is an active anaerobic hydrogen production in Frankia R43 and that this hydrogenase is immunologically related to the subunit HoxU of Ralstonia eutropha.

摘要

研究了弗兰克氏菌 R43 在不同丙酸浓度下的发酵产氢情况。利用 Western blot 对产氢酶进行了表征。生物反应器研究表明，在 50 小时内生长速度提高了 10 倍。研究表明，弗兰克氏菌 R43 中存在活性的厌氧产氢，并且该产氢酶与 Ralstonia eutropha 的亚基 HoxU 在免疫学上有关。