Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, 100875 Beijing, China.
Fish Shellfish Immunol. 2011 Mar;30(3):902-9. doi: 10.1016/j.fsi.2011.01.012. Epub 2011 Jan 27.
Foxp3 is a T cell-specific transcription factor and plays a key role in the development of Treg cells and in the immune regulatory process during inflammation. Here we report cloning and characterization of the full-length cDNA of Atlantic salmon Foxp3, which possesses a Forkhead domain, a zinc finger domain and a leucine-zipper domain as its counterpart in mammals. Foxp3 is highly expressed in thymus. Furthermore, regulated expression was observed in head kidney cells in response to β-glucan and mitogens (LPS and ConA), and in the head kidney, spleen and liver after intraperitoneal injection of live Aeromonas salmonicida. In addition, transfection of CHSE-214 cells with salmon Foxp3 fused with a C-termial RFP tag, resulted in the expression of the transgene in and close to the nuclei upon stimulation. Taken together, these results suggest the presence of a Foxp3 gene in Atlantic salmon that may be an important transcription factor in immune regulation, and further research may reveal the existence of Treg-like T cells in this species.
Foxp3 是一种 T 细胞特异性转录因子，在 Treg 细胞的发育和炎症过程中的免疫调节中发挥关键作用。在这里，我们报告了大西洋鲑鱼 Foxp3 的全长 cDNA 的克隆和特性，它具有 Forkhead 结构域、锌指结构域和亮氨酸拉链结构域，与哺乳动物中的对应物相同。Foxp3 在胸腺中高度表达。此外，在头肾细胞中观察到对β-葡聚糖和有丝分裂原（LPS 和 ConA）的调节表达，以及在腹腔注射活嗜水气单胞菌后在头肾、脾脏和肝脏中的表达。此外，用与 C 端 RFP 标签融合的鲑鱼 Foxp3 转染 CHSE-214 细胞，在刺激时导致转基因在细胞核内和附近表达。总之，这些结果表明大西洋鲑鱼中存在 Foxp3 基因，它可能是免疫调节中的一个重要转录因子，进一步的研究可能揭示该物种中存在 Treg 样 T 细胞。
