Department of Biochemistry, J N Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, India.
IUBMB Life. 2011 Jan;63(1):26-9. doi: 10.1002/iub.410.
Nonenzymatic glycation of macromolecules, especially proteins, takes place mainly due to hyperglycemia in diabetes mellitus. Increased glycolysis during cancer and inflammation during rheumatoid arthritis also contribute to the process of glycation. As lysine residues of proteins are a potential site for glycation, it could be used as a marker for early glycation induced changes in lysine-rich proteins. In the present study, a lysine polymer was incubated with increasing concentrations of glucose for 24 h, and the early glycation product was evaluated by nitroblue tetrazolium assay. The modified polymer together with unmodified one was characterized by gel electrophoresis and UV, fluorescence spectroscopy. Results of the study clearly demonstrate that structural perturbation in the lysine polymer was caused by the early glycation. Further study on detection of antibodies against theglycated proteins in diseased patients might be helpful in early diagnosis of the disease.
非酶糖化的大分子，特别是蛋白质，主要发生在高血糖的糖尿病。增加糖酵解在癌症和炎症类风湿关节炎也有助于糖化过程。作为赖氨酸残基的蛋白质是一个潜在的糖化位点，它可以作为一个标记，用于早期糖化诱导赖氨酸丰富的蛋白质的变化。在本研究中，一个赖氨酸聚合物孵育与葡萄糖浓度的增加 24 小时，和早期糖化产物进行评估硝基四氮唑蓝试验。改性聚合物与未改性的一个特点是凝胶电泳和紫外线、荧光光谱。研究结果清楚地表明，结构的扰动在赖氨酸聚合物是由早期糖化。进一步研究检测抗体对疾病患者的糖基化蛋白可能有助于早期诊断疾病。
