[Study of immunological activity of Aspergillus flavus Link. II. Immunogenicity, antigenic properties and evaluation of cross reactions of Aspergillus flavus antibodies with Aspergillus fumigatus antigens].

The aim of this study was to determine immunogenic and antigenic properties of tested strain. Chemically characterised AEM and APP antigens were used for immunisation of laboratory animals. Immunogenic, antigenic and multideterminant character of tested preparations was shown by immunodiffusion technique. It was found that AEM and its two fractions AS and API share common immunodeterminant what requires additional studies on their further separation. Using techniques described in this study no antigenic relationship was found between tested A. flavus strain and antigens derived from A. fumigatus and A. candidus. Multideterminant character of AEM and APP was confirmed by rockett immunoelectrophoresis, defining 6 different immunodeterminants for APP and 9-10 determinants for AEM, showing by the method used as separate precipitation arcs. In this study a fused rockett immunoelectrophoresis was highly appreciated as an usefull technique for investigation of fungal antigens. The results obtained require further confirmation by testing human sera. They indicate that it would be usefull to apply other antigens than derived from A. fumigatus and A. candidus for clinical diagnostic of Aspergillosis and related diseases.