Institute for Science & Technology in Medicine, Keele University, England, UK.
Curr Eye Res. 2011 Feb;36(2):88-93. doi: 10.3109/02713683.2010.512407.
To determine if microtopographical cues can influence the orientation and extracellular matrix production of human keratocytes in vitro.
Human keratocytes were cultured on grooved and ungrooved polycaprolactone films for up to 3 weeks. The cell morphology was examined using ordinary light microscopy, reflective microscopy, and scanning electron microscopy. The cells gene expression was examined using a GEArray Q series gene expression kit.
Cells initially appeared to orientate in the direction of the grooves. Cells cultured on ungrooved films exhibited random orientations. For longer culture periods on grooved membranes, a second cell layer formed on top of the initial layer at an angle orientation to the initial layer. Analysis of mRNA showed that several genes involved in the production of integrins and matrix metalloproteinases were either up-regulated or down-regulated in the presence of the grooves.
The introduction of microtopographical cues has been shown to influence the orientation of keratocytes and alter their gene expression. This pilot study reveals some important findings that can be used in the development of bioengineered corneas.
确定微观形貌线索是否会影响体外人角膜细胞的取向和细胞外基质的产生。
人角膜细胞在有槽和无槽聚己内酯膜上培养长达 3 周。使用普通光学显微镜、反射显微镜和扫描电子显微镜检查细胞形态。使用 GEArray Q 系列基因表达试剂盒检查细胞的基因表达。
细胞最初似乎沿槽的方向定向。在无槽膜上培养的细胞表现出随机取向。在有槽膜上培养更长时间后，初始层的顶部会以与初始层成一定角度的方式形成第二层细胞。mRNA 分析表明，在存在槽的情况下，几个参与整合素和基质金属蛋白酶产生的基因被上调或下调。
已经证明微观形貌线索的引入会影响角膜细胞的取向并改变其基因表达。这项初步研究揭示了一些重要的发现，可用于生物工程角膜的开发。
