Wright W D, Higashikubo R, Roti Roti J L
Section of Cancer Biology, Washington University School of Medicine, St. Louis, Missouri 63108.
Methods Cell Biol. 1990;33:353-62. doi: 10.1016/s0091-679x(08)60538-5.
Fluorescence assays can be used to reveal molecular interactions through rapidly demonstrable particle-associated events. The additional fact that in many cases fluorescent particles may be analyzed on a per-event basis lends credence to such techniques as probes for biologically significant perturbations and their resolution. Perhaps more importantly, the sorting capability of the flow cytometer enables detailed study of these events in cells in relation to their positions in the cell cycle. Further studies on the effect of drugs and other modalities on the organization of the genome and the nuclear matrix should prove of interest because the interactions of chromatin and this subnuclear particle could be predictive of the state of DNA metabolism under such conditions. With the additional ability of following such organizational changes through the cell cycle, the mechanisms of reversal of perturbing events might be elucidated.
荧光测定法可用于通过快速可证明的与颗粒相关的事件来揭示分子相互作用。在许多情况下，可以基于每个事件对荧光颗粒进行分析，这一额外事实使得此类技术作为生物显著扰动及其解析的探针更具可信度。也许更重要的是，流式细胞仪的分选能力能够详细研究细胞中这些事件与其在细胞周期中的位置之间的关系。关于药物和其他方式对基因组和核基质组织的影响的进一步研究应该会很有趣，因为染色质与这种亚核颗粒的相互作用可能预示着在这种条件下DNA代谢的状态。随着在细胞周期中跟踪此类组织变化的额外能力，可能会阐明干扰事件逆转的机制。
