Nishino N, Scully M F, Rampling M W, Kakkar V V
Thrombosis Research Institute, Chelsea, London.
Thromb Haemost. 1990 Dec 28;64(4):550-5.
A perfusion circuit was designed to investigate in vitro some of the factors which may influence the success of thrombolytic treatment in vivo. The rate of lysis of clotted plasma and different types of artificial thrombi (fibrin thrombi or whole blood thrombi) was measured in citrated plasma or whole blood under static conditions or under shear stress equivalent to the arterial or venous circulation. With both streptokinase (SK) and tissue-type plasminogen activator (t-PA) the rate of lysis of fibrin thrombi and whole blood thrombi was reduced significantly, when compared to the conventional plasma gel clot model (25-fold and 8-fold, respectively). This occurred particularly with SK which showed a reduction (4-fold) in potency relative to t-PA under these conditions. Lysis of thrombi by both activators was observed to be faster in plasma than whole blood, and also faster with whole blood thrombi than fibrin thrombi. High shear stress, generally, caused a reduction in the rate of lysis of fibrin thrombi and an increase in the rate of lysis of whole blood thrombi compared to lysis rates under static conditions. Under all conditions of flow the lysis rate observed at 50 units t-PA per ml was much faster than that at 500 units per ml unlike the conventional plasma gel clot model.
设计了一种灌注回路，用于在体外研究一些可能影响体内溶栓治疗成功的因素。在枸橼酸盐血浆或全血中，于静态条件下或在相当于动脉或静脉循环的剪切应力下，测量凝固血浆和不同类型人工血栓（纤维蛋白血栓或全血血栓）的溶解速率。与传统的血浆凝胶凝块模型相比，使用链激酶（SK）和组织型纤溶酶原激活剂（t-PA）时，纤维蛋白血栓和全血血栓的溶解速率均显著降低（分别降低25倍和8倍）。在这些条件下，SK相对于t-PA的效力降低（4倍），这种情况尤其明显。观察到两种激活剂对血栓的溶解在血浆中比在全血中更快，并且对全血血栓的溶解比对纤维蛋白血栓更快。一般来说，与静态条件下的溶解速率相比，高剪切应力导致纤维蛋白血栓的溶解速率降低，全血血栓的溶解速率增加。在所有流动条件下，每毫升50单位t-PA时观察到的溶解速率比每毫升500单位时快得多，这与传统的血浆凝胶凝块模型不同。
