Molekulare Botanik, Universität Ulm, Ulm, Germany.
FEBS Lett. 2011 Feb 18;585(4):700-4. doi: 10.1016/j.febslet.2011.01.037. Epub 2011 Feb 1.
We here identify the PPR protein MEF14 of the DYW subclass as a specific trans-factor required for C to U editing of site matR-1895 by genetic mapping of an EMS induced editing mutant in Arabidopsis thaliana. The wild type Col MEF14 gene complements mutant protoplasts. A T-DNA insertion in the MEF14 gene abolishes detectable editing at the matR-1895 site. Lack of RNA editing at the matR-1895 site does not alter the level of mature and precursor nad1 mRNA molecules. Such RNA editing mutants can be used to analyse the function of genes like this maturase related reading frame in plant mitochondria.
我们在这里确定 DYW 亚类的 PPR 蛋白 MEF14 是一个特定的反式因子，通过对拟南芥 EMS 诱导的编辑突变体的遗传作图，该因子是 C 到 U 编辑位点 matR-1895 所必需的。野生型 Col MEF14 基因互补突变体原生质体。MEF14 基因中的 T-DNA 插入消除了 matR-1895 位点可检测的编辑。matR-1895 位点的 RNA 编辑缺失不会改变成熟和前体 nad1 mRNA 分子的水平。这种 RNA 编辑突变体可用于分析与植物线粒体成熟酶相关的阅读框等基因的功能。
