下调 Bax 相互作用因子 1 在人胰腺导管腺癌中的表达。
Down-regulation of Bax-interacting factor 1 in human pancreatic ductal adenocarcinoma.
机构信息
Department of Anatomic Pathology, University of South Florida, Tampa, FL, USA.
出版信息
Pancreas. 2011 Apr;40(3):433-7. doi: 10.1097/MPA.0b013e318205eb03.
OBJECTIVE
Bax-interacting factor 1 (Bif-1) protein plays a critical role in apoptosis, mitochondrial morphogenesis, and autophagy, and its loss promotes tumorigenesis. The role of Bif-1 in pancreatic cancer has not been studied.
METHODS
We determined Bif-1 expression in 82 human pancreatic ductal adenocarcinomas (PDCs) and in 82 nonmalignant pancreatic specimens (NMPs), using immunohistochemistry and tissue microarray. Bif-1 immunostain was semiquantitatively scored on a scale of 0 to 9.
RESULTS
Bif-1 scores in NMP were either 6 or 9, with lower scores in only 19 (23.2%) of 82 NMPs. Low Bif-1 expression (score <6) was found in 37 (45.1%) of 82 PDCs, a proportion significantly greater than that found in NMP (P = 0.005). The expression of Bif-1 was twice as likely to be low in PDC as in NMP (relative risk = 1.95, 95% confidence interval, 1.23-3.09). Kaplan-Meier survival estimates showed no difference in survival between patients with low and high Bif-1 expression (P = 0.21, log-rank test).
CONCLUSIONS
The expression of Bif-1 is downregulated in a subset of PDC. This novel finding is in agreement with the tumor suppressor function of Bif-1. The lack of association between Bif-1 expression and patient survival may be best explained by the complexity of carcinogenesis.
目的
Bax 相互作用因子 1（Bif-1）蛋白在细胞凋亡、线粒体形态发生和自噬中发挥关键作用，其缺失可促进肿瘤发生。Bif-1 在胰腺癌中的作用尚未被研究过。
方法
我们通过免疫组织化学和组织微阵列检测了 82 例人胰腺导管腺癌（PDC）和 82 例非恶性胰腺标本（NMP）中的 Bif-1 表达。Bif-1 免疫染色评分范围为 0 至 9。
结果
NMP 的 Bif-1 评分要么为 6，要么为 9，只有 19 例（23.2%）NMP 的评分较低。在 82 例 PDC 中，有 37 例（45.1%）存在低 Bif-1 表达（评分<6），这一比例明显高于 NMP（P=0.005）。Bif-1 在 PDC 中的表达比在 NMP 中低两倍的可能性（相对风险=1.95，95%置信区间，1.23-3.09）。Kaplan-Meier 生存估计显示，低和高 Bif-1 表达患者的生存无差异（P=0.21，对数秩检验）。
结论
Bif-1 的表达在部分 PDC 中下调。这一新发现与 Bif-1 的肿瘤抑制功能一致。Bif-1 表达与患者生存之间缺乏关联，这可能最好用致癌作用的复杂性来解释。
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