[利用重组大肠杆菌通过生物转化生产琥珀酸的再利用]
[Reuse of recombinant Escherichia coli to produce succinic acid by bioconversion].
作者信息
Xu Bing, Jiang Min, Ma Jiangfeng, Liu Shuwen, Hou Guwei, Sui Shanshan
机构信息
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2010 Nov;26(11):1526-31.
The possibility of reusing Escherichia coli cells from the broth for succinic acid production was investigated. Using succinic acid yield and productivity as criterion, we investigated the effects of cell concentration, initial glucose concentration, different neutralizers on the bioconversion. The results revealed that E. coli could convert glucose to succinic acid in a water solution of glucose and a neutralizer. According to the results, the optimal condition was as follows: the cell concentration was 50 (OD600), glucose concentration was 40 g/L and neutralizer was MgCO3. Under the optimum conditions, we carried out the consecutive batch bioconversion in 7 L fermenter. Succinic acid yield reached 91% with the productivity of 3.22 g/(L x h) for the first conversion. For the second conversion, succinic acid yield reached 86% with productivity of 2.04 g/(L x h). Furthermore, we achieved a high mass yield above 83% with the productivity of 1.82 g/(L x h) for the third bioconversion.
研究了从发酵液中重复利用大肠杆菌细胞生产琥珀酸的可能性。以琥珀酸产量和生产率为标准，研究了细胞浓度、初始葡萄糖浓度、不同中和剂对生物转化的影响。结果表明，大肠杆菌能够在葡萄糖和中和剂的水溶液中将葡萄糖转化为琥珀酸。根据结果，最佳条件如下：细胞浓度为50（OD600），葡萄糖浓度为40 g/L，中和剂为MgCO3。在最佳条件下，我们在7 L发酵罐中进行了连续批次生物转化。第一次转化时，琥珀酸产量达到91%，生产率为3.22 g/(L·h)。第二次转化时，琥珀酸产量达到86%，生产率为2.04 g/(L·h)。此外，第三次生物转化时，我们实现了高于83%的高质量产量，生产率为1.82 g/(L·h)。
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