Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada.
J Fish Biol. 2011 Feb;78(2):602-23. doi: 10.1111/j.1095-8649.2010.02881.x. Epub 2011 Jan 11.
The genetic architecture underlying variation in embryonic developmental rate (DR) and genetic covariation with age of maturation (MAT) was investigated in rainbow trout Oncorhynchus mykiss. Highly significant additive parental effects and more limited evidence of epistatic effects on progeny hatching time were detected in three diallel sets of families. Genome scans with an average of 142 microsatellite loci from all 29 linkage groups in two families detected significant quantitative trait loci (QTL) for developmental rate on RT-8 and RT-30 with genome-wide and chromosome-wide effects, respectively. The QTL on linkage group RT-8 explained 23·7% of the phenotypic variation and supports results from previous studies. The co-localization of QTL for both DR and MAT to several linkage groups and the observation that alleles associated with faster developmental rate were found significantly more often in early maturing rather than typical and later maturing male ancestors supports the hypothesis of genetic covariation between DR and MAT. The maturation background and schedule of additional sires, however, did not have a consistent association with their progeny hatching times, suggesting that other genetic, environmental and physiological effects contribute to variation in these life-history traits.
本研究调查了虹鳟（Oncorhynchus mykiss）胚胎发育速率（DR）和与成熟年龄（MAT）遗传协变的遗传结构。在三个完全双列杂交家系中，检测到了高度显著的加性亲本效应和对后代孵化时间的有限的上位性效应证据。在两个家系的 29 个连锁群中的平均 142 个微卫星标记的基因组扫描中，在 RT-8 和 RT-30 上检测到了具有全基因组和染色体-wide 效应的显著数量性状基因座（QTL），用于发育速率。在连锁群 RT-8 上的 QTL 解释了 23.7%的表型变异，与之前的研究结果一致。DR 和 MAT 的 QTL 共同定位于几个连锁群，以及与较快发育速率相关的等位基因在早期成熟而不是典型和晚期成熟的雄性祖先中更频繁地被发现的观察结果，支持了 DR 和 MAT 之间遗传协变的假说。然而，额外父本的成熟背景和计划与它们的后代孵化时间没有一致的关联，这表明其他遗传、环境和生理效应也会导致这些生活史特征的变异。
