Carbini L A, Muñoz Maines V J, Salvaterra P M
Division of Neuroscience, Beckman Research Institute, City of Hope, Duarte, CA 91010.
Neurochem Res. 1990 Nov;15(11):1089-96. doi: 10.1007/BF01101709.
We have measured the steady state levels of choline acetyltransferase (ChAT, EC 2.3.1.6) mRNA during different developmental stages of Drosophila melanogaster using a ChAT specific cRNA probe. ChAT mRNA was first detected approximately 6-7 h after oviposition, increased until the 1st-2nd larval instar, decreased into early pupal stages and increased again during late pupation, reaching a maximum in adults. Northern analysis showed a major RNA band with a Mr of 4.7 kilobases and Western analysis also showed a single major 75 kD protein band at all developmental stages. Our results support the hypothesis that a major point of regulation of ChAT expression may be at the transcriptional level.
我们使用胆碱乙酰转移酶（ChAT，EC 2.3.1.6）特异性cRNA探针，测量了黑腹果蝇不同发育阶段胆碱乙酰转移酶（ChAT）mRNA的稳态水平。ChAT mRNA在产卵后约6-7小时首次被检测到，在1-2龄幼虫期之前增加，在蛹早期减少，并在化蛹后期再次增加，在成虫期达到最大值。Northern分析显示有一条主要的RNA带，分子量为4.7千碱基，Western分析也显示在所有发育阶段都有一条单一的主要75 kD蛋白带。我们的结果支持这样的假说，即ChAT表达调控的一个主要点可能在转录水平。
