Wake Forest Institute for Regenerative Medicine, Medical Center Boulevard, Winston Salem, NC 27154-1094, USA.
J Mater Sci Mater Med. 2011 Mar;22(3):741-51. doi: 10.1007/s10856-011-4242-6. Epub 2011 Feb 1.
To determine whether the use of multiple layers of acellular bladder matrix (ABM) is more suitable for the treatment of abdominal wall hernia than a single layered ABM. The feasibility, biocompatibility and mechanical properties of both materials were assessed and compared. Biocompatibility testing was performed on 4 and 1 layered ABM. The matrices were used to repair an abdominal hernia model in 24 rabbits. The animals were followed for up to 3 months. Immediately after euthanasia, the implant site was inspected and samples were retrieved for histology, scanning electron microscopy and biomechanical studies. Both acellular biomaterials demonstrated excellent biocompatibility. At the time of retrieval, there was no evidence of infection. The matrices demonstrated biomechanical properties comparable to native tissue. Three hernias (25%) were found in the single layer ABM group and only 1 hernia (8%) was found in the 4 layer ABM group. Histologically, the matrix structure was intact and the cell density within the matrices decreased with time. The dominant cell type present within the matrices shifted from lymphocytes to fibroblasts over time. Both ABMs maintained adequate strength over time when used for hernia repair, and there was an extremely low incidence of adhesion formation. The single layer ABM showed enhanced cellular integration, while the 4 layer ABM reduced hernia formation. Either of these matrices may be useful as an off-the-shelf biomaterial for patients requiring fascial repair.
为了确定使用多层去细胞膀胱基质 (ABM) 是否比单层 ABM 更适合治疗腹壁疝，评估并比较了这两种材料的可行性、生物相容性和机械性能。对 4 层和 1 层 ABM 进行了生物相容性测试。将基质用于修复 24 只兔子的腹壁疝模型。对动物进行了长达 3 个月的随访。在安乐死后立即检查植入部位，并取回样本进行组织学、扫描电子显微镜和生物力学研究。两种去细胞生物材料均表现出优异的生物相容性。在回收时，没有感染的证据。基质表现出与天然组织相当的生物力学性能。在单层 ABM 组中发现了 3 个疝（25%），而在 4 层 ABM 组中仅发现了 1 个疝（8%）。组织学上，基质结构完整，随着时间的推移，基质内的细胞密度降低。随着时间的推移，基质内的主要细胞类型从淋巴细胞转变为成纤维细胞。这两种 ABM 在用于疝修复时都能保持足够的强度，并且粘连形成的发生率极低。单层 ABM 显示出增强的细胞整合，而 4 层 ABM 减少了疝的形成。这些基质中的任何一种都可能作为一种现成的生物材料，用于需要筋膜修复的患者。
