Egorov N S, Baranova I P, Khodzhaev M N
Antibiot Khimioter. 1990 Nov;35(11):8-10.
The drug nisin produced by the lactic acid bacteria S. lactis, strain MSU, was identified and described. After 18-hour cultivation of the strain the fermentation broth was centrifuged. The centrifugate contained at an average 2000 IU/ml of the antibiotic. It was purified on silica gel C-3, the eluate was lyophilized and the dry substance was studied by disk electrophoresis in 20 per cent PAAG in the presence of sodium dodecylsulfate. It was found that S. lactis, strain MSU, produced a polypeptide component of the molecular weight of 7000 D. Its electrophoretic mobility corresponded to that of nisaplin. Therefore, nisin was shown to be identical to nisaplin.
对乳酸乳球菌MSU菌株产生的乳链菌肽进行了鉴定和描述。该菌株培养18小时后，将发酵液离心。离心液中抗生素的平均含量为2000国际单位/毫升。将其在硅胶C-3上纯化，洗脱液冻干，所得干物质在含十二烷基硫酸钠的20%聚丙烯酰胺凝胶中进行圆盘电泳分析。结果发现，乳酸乳球菌MSU菌株产生了一种分子量为7000道尔顿的多肽成分。其电泳迁移率与乳链菌肽相同。因此，证明乳链菌肽与乳链菌肽相同。
