Zhao Shu-Min, Zhang Su-Hua, Li Cheng-Tao
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China.
Fa Yi Xue Za Zhi. 2010 Oct;26(5):343-8, 356.
To develop a multiplex PCR system, using insertion/deletion (InDel) polymorphism markers, for forensic DNA identification among Han, Hui, Uighur, Mongolian and Tibetan populations in China.
Highly polymorphic InDel markers from human autosomes were selected using the Human Genome Browser in Galaxy system and dbSNP database. Multiplex PCR primer pairs of selected InDel markers were designed using Primer 3 software. The multiplex PCR system was developed using a five fluorescence dye labeling system. Genetic polymorphisms of selected InDel markers were investigated using the multiplex PCR system among five populations in China.
A new multiplex genotyping system, named InDel_typer30, was successfully developed and validated in this study. The InDel_typer30 system consisted of 30 highly polymorphic InDel markers and 1 Amelogenin gender marker. The average expected heterozygosity of the 30 InDel markers was 0.464, 0.460, 0.453, 0.466 and 0.469 for the Han, Hui, Uighur, Mongolian and Tibetan populations, respectively. The average discrimination power was 0.595, 0.585, 0.586, 0.589 and 0.595 for the Han, Hui, Uighur, Mongolian and Tibetan populations, respectively. The cumulative discrimination power (CDP) were all above 0.999 999 999 996 for the 5 populations.
InDel_typer30 was a useful forensic DNA identification tool for human identification among Han, Hui, Uighur, Mongolian and Tibetan populations in China.
开发一种利用插入/缺失（InDel）多态性标记的多重PCR系统，用于中国汉族、回族、维吾尔族、蒙古族和藏族人群的法医DNA鉴定。
利用Galaxy系统中的人类基因组浏览器和dbSNP数据库，从人类常染色体中选择高度多态性的InDel标记。使用Primer 3软件设计所选InDel标记的多重PCR引物对。采用五荧光染料标记系统开发多重PCR系统。利用该多重PCR系统对中国五个群体中所选InDel标记的遗传多态性进行研究。
本研究成功开发并验证了一种名为InDel_typer30的新型多重基因分型系统。InDel_typer30系统由30个高度多态性的InDel标记和1个牙釉蛋白性别标记组成。这30个InDel标记在汉族、回族、维吾尔族、蒙古族和藏族人群中的平均期望杂合度分别为0.464、0.460、0.453、0.466和0.469。汉族、回族、维吾尔族蒙古族和藏族人群的平均鉴别力分别为0.595、0.585、0.586、0.589和0.595。这5个群体的累积鉴别力（CDP）均高于0.999 999 999 996。
InDel_typer30是一种用于中国汉族、回族、维吾尔族、蒙古族和藏族人群个体识别的有用法医DNA鉴定工具。
