Altmann H
Institute of Biology, Research Center Seibersdorf, Austria.
Acta Biol Hung. 1990;41(1-3):19-34.
Poly(ADP-ribose)-polymerase is an important cellular regulatory enzyme which can change chromatin structure and function. Action mechanisms and activation of the enzyme are described. The synthesis of poly(ADP-ribose) can be modulated by interaction of substances with the DNA binding site of the poly(ADP-ribose)-polymerase. The involvement of this enzyme in DNA repair, differentiation, carcinogenesis and DNA replication has been suggested. Unscheduled DNA synthesis in spleen cells of C57bl mice drug treated and gamma irradiated in vivo and 3 days later UV irradiated in vitro showed a slight decrease in grain numbers of treated animals. Poly(ADP-ribose)-synthesis was highest in the irradiated groups 18 hours after gamma irradiation. A higher amount of supercoils in DNA was generated by both drugs used. In one long-term experiment the gamma-irradiated group of mice had the highest incidence of lymphomas, while the combined treatment group, modulated and gamma irradiated, showed a lymphoma level like in the unirradiated control group.
聚（ADP-核糖）聚合酶是一种重要的细胞调节酶，它可以改变染色质的结构和功能。本文描述了该酶的作用机制和激活过程。聚（ADP-核糖）的合成可通过物质与聚（ADP-核糖）聚合酶的DNA结合位点相互作用来调节。有人提出该酶参与DNA修复、分化、致癌作用和DNA复制。对体内经药物处理和γ射线照射、3天后体外紫外线照射的C57bl小鼠脾细胞进行非预定DNA合成检测，结果显示处理组动物的颗粒数略有减少。γ射线照射后18小时，照射组的聚（ADP-核糖）合成最高。所使用的两种药物均能使DNA产生更高数量的超螺旋。在一项长期实验中，γ射线照射的小鼠组淋巴瘤发病率最高，而经调节和γ射线照射的联合治疗组的淋巴瘤水平与未照射的对照组相似。
