Department of Cardiology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium.
Eur J Appl Physiol. 2011 Sep;111(9):2375-9. doi: 10.1007/s00421-011-1843-1. Epub 2011 Feb 3.
Exercise training improves endothelial function in patients with chronic heart failure (CHF) through functional enhancement of circulating angiogenic cells and increased numbers of circulating endothelial progenitor cells (EPC). In contrast to healthy subjects, an immediate effect of acute exercise on CD34(+)/KDR(+) EPC is absent in CHF. Whether this reflects an attenuated or rather delayed mobilization, is addressed in the present study by measuring CD34(+)/KDR(+) EPC over a longer time period post-exercise. Seven CHF patients and eight healthy subjects (HS; 4 young and 4 age-matched subjects) underwent graded exercise testing (GXT). Venous blood was sampled before and 10, 30, and 60 min, 2, 4, 8, 12, 24 and 48 h following GXT to determine numbers of circulating CD34(+)/KDR(+) EPC (flow cytometry) and serum levels of stromal cell-derived factor (SDF)-1α (ELISA). In both HS groups, CD34(+)/KDR(+) EPC numbers increased within 10 min following GXT and remained elevated for up to 2 h. In CHF patients, the initial increase was small and normalized within 30 min. Evolution of CD34(+)/KDR(+) EPC numbers over time following GXT overall was attenuated in CHF versus HS (p = 0.036). Exercise considerably influenced SDF-1α levels over time (p = 0.0008), without a relation to the changes in CD34(+)/KDR(+) EPC. The immediate effect of acute exercise on CD34(+)/KDR(+) EPC numbers is not delayed, but significantly attenuated in CHF patients compared to HS.
运动训练通过循环血管生成细胞的功能增强和循环内皮祖细胞 (EPC) 数量的增加来改善慢性心力衰竭 (CHF) 患者的内皮功能。与健康受试者不同，急性运动对 CHF 患者 CD34(+)/KDR(+)EPC 的即时效应缺失。本研究通过在运动后较长时间内测量 CD34(+)/KDR(+)EPC，来确定这是否反映了动员的减弱或延迟。7 例 CHF 患者和 8 例健康对照者（HS；4 例年轻和 4 例年龄匹配的受试者）进行了分级运动测试（GXT）。在 GXT 前后以及 GXT 后 10、30 和 60 min、2、4、8、12、24 和 48 h 采集静脉血，以通过流式细胞术测定循环 CD34(+)/KDR(+)EPC 的数量和基质细胞衍生因子 (SDF)-1α 的血清水平（ELISA）。在两个 HS 组中，CD34(+)/KDR(+)EPC 数量在 GXT 后 10 min 内增加，并在 2 h 内保持升高。在 CHF 患者中，初始增加较小，并在 30 min 内恢复正常。与 HS 相比，CHF 患者 GXT 后 CD34(+)/KDR(+)EPC 数量的时间演变总体上减弱（p = 0.036）。运动对 SDF-1α 水平的影响随时间而变化（p = 0.0008），与 CD34(+)/KDR(+)EPC 的变化无关。与 HS 相比，CHF 患者急性运动对 CD34(+)/KDR(+)EPC 数量的即时效应不仅没有延迟，反而明显减弱。
