Universität Leipzig-Herzzentrum, Klinik für Kardiologie, Leipzig, Germany.
J Appl Physiol (1985). 2009 Dec;107(6):1943-50. doi: 10.1152/japplphysiol.00532.2009. Epub 2009 Oct 1.
Exercise stimulates the release of hematopoietic and endothelial progenitor cells (EPC) from the bone marrow. However, no data are available concerning the time frame of EPC release during strenuous exercise. The aim of the present study was to investigate the time-dependent release of progenitor cells during strenuous exercise. Eighteen healthy young men cycled for 4 h continuously at 70% of their individual anaerobic threshold. Peripheral blood was drawn at 16 predefined time points during and after finishing cycling. A significant rise in heart rate and leukocytes was obvious, whereas lactate levels and hematocrit did not change. The amount of circulating progenitor cells, EPCs, mature endothelial cells (mECs), and microparticles, quantified by flow cytometry, showed a significant time-dependent increase at 210/240 min. In addition a very early rise in VEGF and later increase in IL-6, both measured by ELISA, were evident. All observed changes were normalized 24 h after finishing the test. In conclusion, strenuous activity in healthy individuals leads to a time-dependent increase in mECs, PCs, and EPCs that may be related to VEGF and IL-6.
运动可刺激骨髓中造血和内皮祖细胞（EPC）的释放。然而，关于剧烈运动期间 EPC 释放的时间框架尚缺乏数据。本研究旨在探讨剧烈运动期间祖细胞的时间依赖性释放。18 名健康年轻男性以 70%个体无氧阈持续连续骑行 4 小时。在骑行过程中和结束后，在 16 个预先设定的时间点抽取外周血。明显的心率和白细胞升高，而乳酸水平和血细胞比容没有变化。通过流式细胞术定量的循环祖细胞、EPC、成熟内皮细胞（mEC）和微颗粒显示出在 210/240 分钟时呈显著的时间依赖性增加。此外，通过 ELISA 检测到 VEGF 的早期升高和随后的 IL-6 升高。所有观察到的变化在测试结束后 24 小时内恢复正常。总之，健康个体的剧烈活动导致 mEC、PC 和 EPC 的时间依赖性增加，这可能与 VEGF 和 IL-6 有关。
