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基于核核糖体DNA内转录间隔区一级和二级结构研究的裂叶藓属(苔藓植物门,灰藓科)系统发育研究

[The phylogeny of Schistidium (Bryophyta, Grimmiaceae) based on primary and secondary structure study of nuclear rDNA internal transcribed spacers].

作者信息

Miliutina I A, Goriunov D V, Ignatov M S, Ignatova E A, Troitskiĭ A V

出版信息

Mol Biol (Mosk). 2010 Nov-Dec;44(6):994-1009.

PMID:21290822
Abstract

The phylogeny of Schistidium (Bryophyta, Grimmiaceae) was studied on the basis of nucleotide sequences of internal transcribed spacers ITS1-2 of nuclear DNA and trnT-trnD region of chloroplast DNA. The consistency of phylogenetic trees constructed from nuclear and chloroplast sequences was shown. A basal grade and two large clades were resolved on the phylogenetic trees. Morphological characteristics specific for these clades were described. ITS1 and ITS2 secondary structures of Schistidium species were modeled using thermodynamic criteria. Four different structures of the longest ITS1 hairpin were identified. Possible paths of Schistidium evolution were considered based on the four types of ITS1 secondary structure and phylogenetic trees.

摘要

基于核DNA的内部转录间隔区ITS1-2和叶绿体DNA的trnT-trnD区域的核苷酸序列,对裂萼藓属(苔藓植物门,灰藓科)的系统发育进行了研究。结果表明,由核序列和叶绿体序列构建的系统发育树具有一致性。在系统发育树上解析出一个基部类群和两个大的分支。描述了这些分支特有的形态特征。利用热力学标准对裂萼藓属物种的ITS1和ITS2二级结构进行了建模。鉴定出最长ITS1发夹的四种不同结构。基于ITS1二级结构的四种类型和系统发育树,探讨了裂萼藓属可能的进化路径。

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