Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Lett Appl Microbiol. 2011 May;52(5):448-55. doi: 10.1111/j.1472-765X.2011.03019.x. Epub 2011 Feb 24.
To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol.
The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria was investigated in batch experiments with various concentrations of phenol. The bacterial cells were immobilized by attachment to or encapsulation in silica. The encapsulated bacteria had the highest phenol degradation rate, especially at initial phenol concentrations between 7500 and 10000 mg l(-1) . Additionally, the immobilized cells could continuously degrade phenol for up to 55 days.
The encapsulation of a mixed culture of Methylobacterium sp. NP3 and Acinetobacter sp. PK1 is an effective and easy technique that can be used to improve bacterial stability and phenol degradation.
Wastewater from various industries contains high concentrations of phenol, which can cause wastewater treatment failure. Silica-immobilized bacteria could be applied in bioreactors to initially remove the phenol, thereby preventing phenol shock loads to the wastewater treatment system.
将甲基杆菌（Methylobacterium sp.）NP3 和不动杆菌（Acinetobacter sp.）PK1 固定在硅胶上，并测定固定化细菌降解高浓度苯酚的能力。
采用批次实验，研究了悬浮和固定化甲基杆菌（Methylobacterium sp.）NP3 和不动杆菌（Acinetobacter sp.）PK1 细菌在不同浓度苯酚条件下的苯酚降解活性。通过附着或包封在硅胶上对细菌细胞进行固定化。包封细菌具有最高的苯酚降解率，尤其是在初始苯酚浓度为 7500 至 10000mg/L 之间时。此外，固定化细胞可连续降解苯酚长达 55 天。
固定化混合培养的甲基杆菌（Methylobacterium sp.）NP3 和不动杆菌（Acinetobacter sp.）PK1 是一种有效且简单的技术，可用于提高细菌稳定性和苯酚降解能力。
来自各种工业的废水含有高浓度的苯酚，这可能导致废水处理失败。硅胶固定化细菌可应用于生物反应器中，以初始去除苯酚，从而防止苯酚对废水处理系统的冲击负荷。
