Key Laboratory for Molecular Biology and Biopharmaceutic, Mianyang Normal University, Mianyang, Sichuan, China.
J Basic Microbiol. 2013 Mar;53(3):224-30. doi: 10.1002/jobm.201100460. Epub 2012 Aug 23.
Strain BS8Y with high biodegradation activity and high tolerance of phenol was isolated from activated sludge in an insulating material plant of China. This strain was capable of removing 99.2% of the initial 600 mg/l phenol in liquid minimal medium within 24 h and tolerating phenol at concentrations of up to 1,200 mg/ml. DNA sequencing and homologous analysis of the 16S rRNA gene identified that the strain BS8Y belonged to an Acinetobacter species. Polyvinyl alcohol was used as gel matrix to immobilize the strain BS8Y. The factors affecting the phenol degradation by immobilized cells and the phenol removal efficiency of free and immobilized cells were investigated; the stability of the immobilized cells is also reported. The results show that the immobilized cells could tolerate a higher phenol level and protected the bacteria much more effectively against changes in temperature and pH. The phenol degradation efficiency was high at up to 96% within 30 h, with an initial concentration of 800 mg/l phenol, and the immobilized cells showed better performance than the suspended cells. Reusability tests revealed that the immobilized cells were stable enough even after reuse for ten times or storing at 4°C for 35 d. These results demonstrate that immobilized Acinetobacter sp. BS8Y possesses a good application potential in the treatment of phenol-containing wastewater.
从中国一家绝缘材料厂的活性污泥中分离到一株具有高生物降解活性和高苯酚耐受能力的 BS8Y 菌株。该菌株能够在 24 小时内去除初始浓度为 600mg/L 的苯酚中的 99.2%，并且能够耐受高达 1200mg/ml 的苯酚。通过 16S rRNA 基因的 DNA 测序和同源性分析，鉴定 BS8Y 菌株属于不动杆菌属。使用聚乙烯醇作为凝胶基质来固定 BS8Y 菌株。研究了固定化细胞降解苯酚的影响因素及其对游离和固定化细胞去除苯酚效率的影响，并报道了固定化细胞的稳定性。结果表明，固定化细胞能够耐受更高的苯酚水平，并且能够更有效地保护细菌免受温度和 pH 值变化的影响。在初始苯酚浓度为 800mg/L 的条件下，30 小时内苯酚降解效率高达 96%，固定化细胞的性能优于悬浮细胞。重复使用实验表明，即使重复使用十次或在 4°C 下储存 35 天，固定化细胞也足够稳定。这些结果表明，固定化不动杆菌 BS8Y 菌株在处理含酚废水方面具有良好的应用潜力。
