血浆铜蓝蛋白作为肥胖的生物标志物：一种蛋白质组学方法。

Plasma ceruloplasmin as a biomarker for obesity: a proteomic approach.

机构信息

Yonsei University Research Institute of Science for Aging, Yonsei University, Seoul, Republic of Korea.

出版信息

Clin Biochem. 2011 Apr;44(5-6):351-6. doi: 10.1016/j.clinbiochem.2011.01.014. Epub 2011 Feb 1.

DOI:10.1016/j.clinbiochem.2011.01.014

PMID:21291874

Abstract

OBJECTIVES

This study aimed to investigate new biomarkers of obesity particularly in relation with inflammation-associated proteins using protein differential display techniques.

DESIGN AND METHODS

Comparison of protein expression in plasma between non-obese (n=109, body mass index, BMI<25kg/m(2)) and obese (n=32, BMI≥25kg/m(2)) groups was carried out using two-dimensional gel electrophoresis (2-DE) analysis. ELISA was also performed for validation.

RESULTS

Among six differentially expressed protein spots, ceruloplasmin (Cp) and fibrinogen were over-expressed in obese group. Plasma Cp levels were significantly higher in obese group than non-obese group (34.0 ± 8.6 vs. 41.3 ± 12.7mg/dL, p<0.001) and positively correlated with age (r=0.253, p<0.005), BMI (r=0.265, p<0.001) and hsCRP (r=0.385, p<0.001). In stepwise multiple linear regression analysis, plasma Cp along with hsCRP were found predictors for obesity (adjusted β-coefficient=0.266, p<0.01).

CONCLUSION

Elevated plasma Cp levels were significantly associated with obesity, which may be suggested to be a marker of obesity.

摘要

目的

本研究旨在使用蛋白质差异显示技术，寻找肥胖相关的新生物标志物，尤其是与炎症相关的蛋白。

设计与方法

采用二维凝胶电泳（2-DE）分析，比较非肥胖（n=109，BMI<25kg/m(2)）和肥胖（n=32，BMI≥25kg/m(2)）两组人群血浆中的蛋白质表达差异。通过酶联免疫吸附试验（ELISA）进行验证。

结果

在 6 个差异表达的蛋白质斑点中，铜蓝蛋白（Cp）和纤维蛋白原在肥胖组中表达上调。肥胖组的血浆 Cp 水平显著高于非肥胖组（34.0±8.6 vs. 41.3±12.7mg/dL，p<0.001），并与年龄（r=0.253，p<0.005）、BMI（r=0.265，p<0.001）和高敏 C 反应蛋白（hsCRP）（r=0.385，p<0.001）呈正相关。在逐步多元线性回归分析中，血浆 Cp 与 hsCRP 一起被发现是肥胖的预测因子（调整β系数=0.266，p<0.01）。