State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Apr;78(4):1305-9. doi: 10.1016/j.saa.2011.01.001. Epub 2011 Jan 12.
Intermolecular interactions of adenosine 5'-triphosphate (ATP) with Lysine (Lys) and Mg(2+) were studied in aqueous solution by (1)H and (31)P NMR spectra. In the metal-free system, the N-1 atom of the purine ring of ATP and carboxyl group of Lys are the interaction sites at low pH conditions. With increasing pH, the interaction efficiency between the phosphate group of ATP and the protonated ammonium group of Lys increased significantly, while that with carboxyl group in Lys decreased. In the Mg(2+)-Lys-ATP system, multi-interactions, such as coordination, cations (Mg(2+), NH(3)(+))-π, hydrogen bonding, ion-pairing interactions and electrostatic interactions co-existed. In addition, the recognition of ATP by the amino acid cation (Lys) was significantly promoted by the addition of magnesium ion, which led to the coordination competition between Lys and ATP.
在水溶液中，通过 (1)H 和 (31)P NMR 光谱研究了三磷酸腺苷 (ATP) 与赖氨酸 (Lys) 和 Mg(2+) 的分子间相互作用。在无金属体系中，ATP 的嘌呤环的 N-1 原子和 Lys 的羧基是在低 pH 条件下的相互作用位点。随着 pH 值的增加，ATP 的磷酸基团与 Lys 的质子化铵基团之间的相互作用效率显著增加，而与 Lys 的羧基之间的相互作用效率则降低。在 Mg(2+)-Lys-ATP 体系中，配位、阳离子 (Mg(2+)、NH(3)(+))-π、氢键、离子对相互作用和静电相互作用等多种相互作用同时存在。此外，镁离子的加入显著促进了氨基酸阳离子 (Lys) 对 ATP 的识别，导致 Lys 与 ATP 之间的配位竞争。
