• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种分拣平台决定了细菌 III 型系统中蛋白质分泌的顺序。

A sorting platform determines the order of protein secretion in bacterial type III systems.

机构信息

Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.

出版信息

Science. 2011 Mar 4;331(6021):1188-91. doi: 10.1126/science.1201476. Epub 2011 Feb 3.

DOI:10.1126/science.1201476
PMID:21292939
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3859126/
Abstract

Bacterial type III protein secretion systems deliver effector proteins into eukaryotic cells in order to modulate cellular processes. Central to the function of these protein-delivery machines is their ability to recognize and secrete substrates in a defined order. Here, we describe a mechanism by which a type III secretion system from the bacterial enteropathogen Salmonella enterica serovar Typhimurium can sort its substrates before secretion. This mechanism involves a cytoplasmic sorting platform that is sequentially loaded with the appropriate secreted proteins. The sequential loading of this platform, facilitated by customized chaperones, ensures the hierarchy in type III protein secretion. Given the presence of these machines in many important pathogens, these findings can serve as the bases for the development of novel antimicrobial strategies.

摘要

细菌 III 型蛋白分泌系统将效应蛋白输送到真核细胞中，以调节细胞过程。这些蛋白输送机器的核心功能是能够按特定顺序识别和分泌底物。在这里，我们描述了一种机制，即来自肠道病原体鼠伤寒沙门氏菌的 III 型分泌系统在分泌前可以对其底物进行分类。该机制涉及一个细胞质分拣平台，该平台依次装载适当的分泌蛋白。定制伴侣蛋白有助于该平台的顺序加载，确保了 III 型蛋白分泌的层次结构。鉴于这些机器存在于许多重要的病原体中，这些发现可以为新型抗菌策略的发展提供基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/5891775c231a/nihms475232f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/a11119307edb/nihms475232f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/0dd2fb055f10/nihms475232f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/9a912fa2867a/nihms475232f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/5891775c231a/nihms475232f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/a11119307edb/nihms475232f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/0dd2fb055f10/nihms475232f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/9a912fa2867a/nihms475232f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/588c/3859126/5891775c231a/nihms475232f4.jpg

相似文献

1
A sorting platform determines the order of protein secretion in bacterial type III systems.一种分拣平台决定了细菌 III 型系统中蛋白质分泌的顺序。
Science. 2011 Mar 4;331(6021):1188-91. doi: 10.1126/science.1201476. Epub 2011 Feb 3.
2
Microbiology. Establishing the secretion hierarchy.微生物学。建立分泌等级体系。
Science. 2011 Mar 4;331(6021):1147-8. doi: 10.1126/science.1203195.
3
A new means to identify type 3 secreted effectors: functionally interchangeable class IB chaperones recognize a conserved sequence.一种鉴定 III 型分泌效应子的新方法：功能可互换的 I 类分子伴侣识别保守序列。
mBio. 2012 Feb 14;3(1). doi: 10.1128/mBio.00243-11. Print 2012.
4
Role of autocleavage in the function of a type III secretion specificity switch protein in Salmonella enterica serovar Typhimurium.自切割在鼠伤寒沙门氏菌III型分泌特异性开关蛋白功能中的作用
mBio. 2015 Oct 13;6(5):e01459-15. doi: 10.1128/mBio.01459-15.
5
Structural Features Reminiscent of ATP-Driven Protein Translocases Are Essential for the Function of a Type III Secretion-Associated ATPase.与ATP驱动的蛋白质转运酶相似的结构特征对于III型分泌相关ATP酶的功能至关重要。
J Bacteriol. 2015 Sep;197(18):3007-14. doi: 10.1128/JB.00434-15. Epub 2015 Jul 13.
6
A polymorphic helix of a Salmonella needle protein relays signals defining distinct steps in type III secretion.沙门氏菌针状蛋白的多态性螺旋传递信号，定义了 III 型分泌过程中的不同步骤。
PLoS Biol. 2019 Jul 1;17(7):e3000351. doi: 10.1371/journal.pbio.3000351. eCollection 2019 Jul.
7
Deletion of invH gene in Salmonella enterica serovar Typhimurium limits the secretion of Sip effector proteins.肠沙门氏菌 Typhimurium 中 invH 基因的缺失限制了 Sip 效应蛋白的分泌。
Microbes Infect. 2013 Jan;15(1):66-73. doi: 10.1016/j.micinf.2012.10.014. Epub 2012 Nov 13.
8
Substrate-engaged type III secretion system structures reveal gating mechanism for unfolded protein translocation.底物结合型 III 型分泌系统结构揭示了未折叠蛋白易位的门控机制。
Nat Commun. 2021 Mar 9;12(1):1546. doi: 10.1038/s41467-021-21143-1.
9
The chaperone binding domain of SopE inhibits transport via flagellar and SPI-1 TTSS in the absence of InvB.在没有InvB的情况下，SopE的伴侣蛋白结合结构域通过鞭毛和SPI-1三型分泌系统抑制转运。
Mol Microbiol. 2006 Jan;59(1):248-64. doi: 10.1111/j.1365-2958.2005.04931.x.
10
Structures of chaperone-substrate complexes docked onto the export gate in a type III secretion system.伴侣蛋白-底物复合物在 III 型分泌系统出口门的对接结构。
Nat Commun. 2018 May 2;9(1):1773. doi: 10.1038/s41467-018-04137-4.

引用本文的文献

1
The predicted sorting platform dynamically associates with the type III secretion system from Xanthomonas euvesicatoria in response to the external pH.预测的分选平台会根据外部pH值与来自黄单胞菌的III型分泌系统动态关联。
BMC Microbiol. 2025 Aug 25;25(1):545. doi: 10.1186/s12866-025-04227-6.
2
analysis of type III secretion chaperone proteins indicates a cytosolic handover of virulence effectors.III型分泌伴侣蛋白分析表明毒力效应蛋白存在胞质交接。
FEMS Microbes. 2025 Jul 31;6:xtaf010. doi: 10.1093/femsmc/xtaf010. eCollection 2025.
3
Interplay between SpaO variants shapes the architecture of the type III secretion sorting platform.

本文引用的文献

1
Biological diversity of prokaryotic type IV secretion systems.原核生物 IV 型分泌系统的生物多样性。
Microbiol Mol Biol Rev. 2009 Dec;73(4):775-808. doi: 10.1128/MMBR.00023-09.
2
The Chlamydia type III secretion system C-ring engages a chaperone-effector protein complex.沙眼衣原体III型分泌系统的C环与一种伴侣-效应蛋白复合物结合。
PLoS Pathog. 2009 Sep;5(9):e1000579. doi: 10.1371/journal.ppat.1000579. Epub 2009 Sep 11.
3
Salmonella enterica serovar typhimurium pathogenicity island 1-encoded type III secretion system translocases mediate intimate attachment to nonphagocytic cells.
SpaO变体之间的相互作用塑造了III型分泌分选平台的结构。
bioRxiv. 2025 May 15:2025.05.15.654217. doi: 10.1101/2025.05.15.654217.
4
Beyond pathogenicity: the immunomodulatory role of the type III secretion system in beneficial plant-microbe interactions.超越致病性：III型分泌系统在有益植物-微生物相互作用中的免疫调节作用
Open Biol. 2025 May;15(5):240318. doi: 10.1098/rsob.240318. Epub 2025 May 28.
5
On the role of the sorting platform in hierarchical type III secretion regulation in enteropathogenic .关于分选平台在肠道致病性细菌Ⅲ型分泌分层调控中的作用
J Bacteriol. 2025 Mar 20;207(3):e0044624. doi: 10.1128/jb.00446-24. Epub 2025 Mar 3.
6
Structure classification of the proteins from Salmonella enterica pangenome revealed novel potential pathogenicity islands.从沙门氏菌泛基因组中蛋白质的结构分类揭示了新的潜在致病性岛。
Sci Rep. 2024 May 28;14(1):12260. doi: 10.1038/s41598-024-60991-x.
7
Cytosolic sorting platform complexes shuttle type III secretion system effectors to the injectisome in Yersinia enterocolitica.细胞质分拣平台复合物将 III 型分泌系统效应蛋白输送到肠炎沙门氏菌的注射器中。
Nat Microbiol. 2024 Jan;9(1):185-199. doi: 10.1038/s41564-023-01545-1. Epub 2024 Jan 3.
8
Blue Native PAGE Analysis of Bacterial Secretion Complexes.Blue Native PAGE 分析细菌分泌复合物。
Methods Mol Biol. 2024;2715:331-362. doi: 10.1007/978-1-0716-3445-5_22.
9
Virulence-associated type III secretion systems in Gram-negative bacteria.革兰氏阴性菌中与毒力相关的 III 型分泌系统。
Microbiology (Reading). 2023 Jun;169(6). doi: 10.1099/mic.0.001328.
10
Recruitment of heterologous substrates by bacterial secretion systems for transkingdom translocation.细菌分泌系统招募异源底物进行跨王国易位。
Front Cell Infect Microbiol. 2023 Mar 6;13:1146000. doi: 10.3389/fcimb.2023.1146000. eCollection 2023.
肠炎沙门氏菌鼠伤寒血清型1型分泌系统转位酶介导与非吞噬细胞的紧密附着。
Infect Immun. 2009 Jul;77(7):2635-42. doi: 10.1128/IAI.00077-09. Epub 2009 Apr 13.
4
The type VI secretion system: translocation of effectors and effector-domains.VI型分泌系统：效应蛋白及效应蛋白结构域的转运
Curr Opin Microbiol. 2009 Feb;12(1):11-7. doi: 10.1016/j.mib.2008.11.010. Epub 2009 Jan 21.
5
Mechanisms of type III protein export for bacterial flagellar assembly.细菌鞭毛组装的III型蛋白质输出机制。
Mol Biosyst. 2008 Nov;4(11):1105-15. doi: 10.1039/b808065h. Epub 2008 Sep 24.
6
Characterization of soluble complexes of the Shigella flexneri type III secretion system ATPase.福氏志贺菌Ⅲ型分泌系统ATP酶可溶性复合物的特性分析
FEMS Microbiol Lett. 2008 Sep;286(2):274-8. doi: 10.1111/j.1574-6968.2008.01284.x. Epub 2008 Jul 23.
7
Impassable YscP substrates and their impact on the Yersinia enterocolitica type III secretion pathway.不可通行的YscP底物及其对小肠结肠炎耶尔森菌III型分泌途径的影响。
J Bacteriol. 2008 Sep;190(18):6204-16. doi: 10.1128/JB.00467-08. Epub 2008 Jul 18.
8
Protein delivery into eukaryotic cells by type III secretion machines.通过III型分泌机制将蛋白质递送至真核细胞。
Nature. 2006 Nov 30;444(7119):567-73. doi: 10.1038/nature05272.
9
Secretion signal recognition by YscN, the Yersinia type III secretion ATPase.耶尔森氏菌III型分泌ATP酶YscN对分泌信号的识别。
Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16490-5. doi: 10.1073/pnas.0605974103. Epub 2006 Oct 18.
10
The type III secretion injectisome.III型分泌注射体
Nat Rev Microbiol. 2006 Nov;4(11):811-25. doi: 10.1038/nrmicro1526.