一种分拣平台决定了细菌 III 型系统中蛋白质分泌的顺序。
A sorting platform determines the order of protein secretion in bacterial type III systems.
机构信息
Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.
出版信息
Science. 2011 Mar 4;331(6021):1188-91. doi: 10.1126/science.1201476. Epub 2011 Feb 3.
Abstract
Bacterial type III protein secretion systems deliver effector proteins into eukaryotic cells in order to modulate cellular processes. Central to the function of these protein-delivery machines is their ability to recognize and secrete substrates in a defined order. Here, we describe a mechanism by which a type III secretion system from the bacterial enteropathogen Salmonella enterica serovar Typhimurium can sort its substrates before secretion. This mechanism involves a cytoplasmic sorting platform that is sequentially loaded with the appropriate secreted proteins. The sequential loading of this platform, facilitated by customized chaperones, ensures the hierarchy in type III protein secretion. Given the presence of these machines in many important pathogens, these findings can serve as the bases for the development of novel antimicrobial strategies.
摘要
细菌 III 型蛋白分泌系统将效应蛋白输送到真核细胞中，以调节细胞过程。这些蛋白输送机器的核心功能是能够按特定顺序识别和分泌底物。在这里，我们描述了一种机制，即来自肠道病原体鼠伤寒沙门氏菌的 III 型分泌系统在分泌前可以对其底物进行分类。该机制涉及一个细胞质分拣平台，该平台依次装载适当的分泌蛋白。定制伴侣蛋白有助于该平台的顺序加载，确保了 III 型蛋白分泌的层次结构。鉴于这些机器存在于许多重要的病原体中，这些发现可以为新型抗菌策略的发展提供基础。
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