从淡紫拟青霉中生产几丁质酶和疏水性蛋白及其活性。
Production and activities of chitinases and hydrophobins from Lecanicillium lecanii.
机构信息
Laboratory of Biopolymers, Biotechnology Department, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco, No. 186, 09340, Mexico City, Mexico.
出版信息
Bioprocess Biosyst Eng. 2011 Aug;34(6):681-6. doi: 10.1007/s00449-011-0517-z. Epub 2011 Feb 4.
The production of chitinases and hydrophobins from Lecanicillium lecanii was influenced by the cultivation method and type of carbon source. Crude enzyme obtained from solid-substrate culture presented activities of exochitinases (32 and 51 kDa), endochitinases (26 kDa), β-N-acetylhexosaminidases (61, 80, 96 and 111 kDa). Additionally, submerged cultures produced exochitinases (32 and 45 kDa), endochitinases (10 and 26 kDa) and β-N-acetylhexosaminidases (61, 96 and 111 kDa). β-N-acetylhexosaminidases activity determined in solid-substrate culture with added chitin was ca. threefold (7.58 ± 0.57 U mg(-1)) higher than submerged culture (2.73 + 0.57 U mg(-1)). Similarly, hydrophobins displayed higher activities in solid-substrate culture (627.3 ± 2 μg protein mL(-1)) than the submerged one (57.4 ± 4.7 μg protein mL(-1)). Molecular weight of hydrophobins produced in solid-substrate culture was 7.6 kDa and they displayed surface activity on Teflon.
从淡紫拟青霉生产几丁质酶和几丁质结合蛋白受培养方法和类型的碳源的影响。从固态发酵获得的粗酶表现出的外切几丁质酶（32 和 51 kDa），内切几丁质酶（26 kDa），β-N-乙酰氨基葡萄糖苷酶（61、80、96 和 111 kDa）的活性。此外，在液体培养下产生外切几丁质酶（32 和 45 kDa），内切几丁质酶（10 和 26 kDa）和β-N-乙酰氨基葡萄糖苷酶（61、96 和 111 kDa）。添加几丁质的固态发酵中β-N-乙酰氨基葡萄糖苷酶活性（7.58±0.57 U mg-1）比液体培养下（2.73+0.57 U mg-1）高约三倍。同样，在固态发酵下几丁质结合蛋白（627.3±2μg 蛋白 mL-1）比液体培养下（57.4±4.7μg 蛋白 mL-1）具有更高的活性。在固态发酵下生产的几丁质结合蛋白的分子量为 7.6 kDa，它们在特氟隆表面具有活性。
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