Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 7558505, Japan.
Neurochem Res. 2011 May;36(5):849-55. doi: 10.1007/s11064-011-0413-6. Epub 2011 Feb 4.
In autoimmune disorders of the peripheral nervous system (PNS), including Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy, breakdown of the blood-nerve barrier (BNB) has been considered to be a key step in the disease process. Although glucocorticoids (GCs) have been shown to effectively restore the blood-brain barrier (BBB) in some inflammatory central nervous system diseases such as multiple sclerosis, their action against the BNB has not yet been examined. To elucidate the role of GCs on the BNB, we established a novel human immortalized endothelial cell lines derived from the BNB. The established cell line termed "DH-BNBs" expresses two important tight junction proteins, claudin-5 and occludin. Using DH-BNBs, we analyzed how GCs affect BNB function. We herein report that GCs up-regulate the expression of claudin-5 and increase the barrier properties of the BNB. This is the first report which indicates how GCs affect the blood-nerve barrier.
在周围神经系统（PNS）的自身免疫性疾病中，包括格林-巴利综合征和慢性炎症性脱髓鞘性多发性神经病，血-神经屏障（BNB）的破坏被认为是疾病进程中的关键步骤。虽然糖皮质激素（GCs）已被证明可有效恢复多发性硬化等一些炎症性中枢神经系统疾病的血脑屏障（BBB），但其对 BNB 的作用尚未得到检验。为了阐明 GCs 对 BNB 的作用，我们建立了一种新型的源自 BNB 的人永生化内皮细胞系。该建立的细胞系称为“DH-BNBs”，表达两种重要的紧密连接蛋白，即 Claudin-5 和 Occludin。使用 DH-BNBs，我们分析了 GCs 如何影响 BNB 功能。我们在此报告 GCs 上调 Claudin-5 的表达并增加 BNB 的屏障特性。这是首个报告 GCs 如何影响血-神经屏障的研究。
