Akman Tarik, Erken Haydar, Acar Goksemin, Bolat Elif, Kizilay Zahir, Acar Feridun, Genc Osman
Pamukkale University, Faculty of Medicine, Department of Neurology, Denizli, Turkey.
Turk Neurosurg. 2011 Jan;21(1):1-5.
Experimental and clinical studies have revealed that hippocampal DBS can control epileptic activity, but the mechanism of action is obscure and optimal stimulation parameters are not clearly defined. The aim was to evaluate the effects of high frequency hippocampal stimulation on cortical epileptic activity in penicillin-induced epilepsy model.
Twenty-five Sprague-Dawley rats were implanted DBS electrodes. In group-1 (n=10) hippocampal DBS was off and in the group-2 (n=10) hippocampal DBS was on (185 Hz, 0.5V, 1V, 2V, and 5V for 60 sec) following penicillin G injection intracortically. In the control group hippocampal DBS was on following 8 μl saline injection intracortically. EEG recordings were obtained before and 15 minutes following penicillin-G injection, and at 10th minutes following each stimulus for analysis in terms of frequency, amplitude, and power spectrum.
High frequency hippocampal DBS suppressed the acute penicillin-induced cortical epileptic activity independent from stimulus intensity. In the control group, hippocampal stimulation alone lead only to diffuse slowing of cerebral bioelectrical activity at 5V stimulation.
Our results revealed that continuous high frequency stimulation of the hippocampus suppressed acute cortical epileptic activity effectively without causing secondary epileptic discharges. These results are important in terms of defining the optimal parameters of hippocampal DBS in patients with epilepsy.
实验和临床研究表明，海马深部脑刺激（DBS）可控制癫痫活动，但其作用机制尚不清楚，最佳刺激参数也未明确界定。本研究旨在评估高频海马刺激对青霉素诱导的癫痫模型中皮质癫痫活动的影响。
25只Sprague-Dawley大鼠植入DBS电极。第1组（n = 10）海马DBS关闭，第2组（n = 10）在脑内注射青霉素G后开启海马DBS（185Hz，0.5V、1V、2V和5V，持续60秒）。对照组在脑内注射8μl生理盐水后开启海马DBS。在注射青霉素G前、注射后15分钟以及每次刺激后第10分钟记录脑电图，分析频率、幅度和功率谱。
高频海马DBS可抑制急性青霉素诱导的皮质癫痫活动，且与刺激强度无关。在对照组中，仅在5V刺激时海马刺激才导致脑生物电活动弥漫性减慢。
我们的结果表明，持续高频刺激海马可有效抑制急性皮质癫痫活动，且不会引起继发性癫痫放电。这些结果对于确定癫痫患者海马DBS的最佳参数具有重要意义。
