Indian Association for the Cultivation of Science, Calcutta 700032, India.
J Biomol Struct Dyn. 2011 Apr;28(5):827-31. doi: 10.1080/07391102.2011.10508610.
tRNAs are mostly transcribed from un-fragmented genes, but occasionally also from split genes, with separated 5' and 3' halves. A reanalysis of the existing data on Staphylothermus marinus and Staphylothermus hellenicus hints of a novel hybrid gene that encodes both an un-fragmented and a 5'-split-half together in one. The corresponding 3' complement-gene is located elsewhere on the genome. As un-fragmented, the hybrid gene transcribes to tRNA(lys)(TTT). But as 5'-half, it trans-splices with its 3'-complement-half to tRNA(lys)(CTT), the tRNA missed so far. This hybrid of the split and the un-fragmented in one suggests a deeper synergy between the two, and hints of co-evolution. Furthermore, in a subtle contrast to the widely held idea of conservation of 3'-half, it is precisely the 3'-half that varies in these two tRNAs; the 5'-half remains conserved.
tRNA 主要由未断裂的基因转录而来，但偶尔也由分裂基因转录，这些基因的 5'和 3'端是分开的。对 Staphylothermus marinus 和 Staphylothermus hellenicus 现有数据的重新分析表明，有一种新的杂合基因，它将未断裂的和 5'端分裂的一半一起编码在一个基因中。相应的 3'互补基因位于基因组的其他位置。作为未断裂的部分，该杂合基因转录为 tRNA(lys)(TTT)。但作为 5'端的一半，它与 3'端的互补部分进行反式拼接，形成迄今为止缺失的 tRNA(lys)(CTT)。这种分裂和未断裂的融合在一个基因中表明两者之间存在更深层次的协同作用，并暗示它们共同进化。此外，与广泛持有的 3'端保守的观点形成微妙对比的是，正是这两个 tRNA 的 3'端发生了变化；5'端保持保守。
